PXD029208

PXD029208 is an original dataset announced via ProteomeXchange.

Title	Novel Regulators of Retina Neovascularization
Description	Mechanisms of vessel remodeling are not yet fully understood. The purpose of this study is to identify proteins that regulate vascular remodeling in an ROP mouse model. Mice were treated with fluctuating oxygen levels and retinas sampled at the peak of vessel regression (PN12) or neovascularization (PN17) for comparative SWATH-MS proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS). We further developed an endothelial cell culture ROP correlate to validate expression of target neovascular markers. 5191 proteins were identified in the retinas of which 498 showed significant changes in expression in elevated oxygen and 345 after a return to normoxia. 122 proteins were uniquely associated with vessel regression and 69 with neovascularization (FC>1.5; P<0.05). Of the latter set, 56 were detected in human retinal endothelial cells (HRECs) of which 42 showed altered expression in the HREC-ROP correlate and 23 regulated in the same direction as in the neovascular retinas. In functional analysis, HREC-specific proteins control angiogenesis-related processes including extracellular matrix remodeling, cell migration and adhesion, junction proteins and proliferation. RNA interference and transfection overexpression studies confirmed that VASP and ECH1, showing highest expression levels in hypoxic HRECs, promoted endothelial cell proliferation while SNX1 and CD109, showing lowest expression, inhibited their proliferation.
HostingRepository	iProX
AnnounceDate	2021-10-18
AnnouncementXML	Submission_2021-10-18_19:38:55.962.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manhong Xu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 19:35:21	ID requested
⏵ 1	2021-10-18 19:38:56	announced

Dataset with its publication pending

submitter keyword: ROP mouse, Retina, SWATH-MS

Xiaorong Li
contact affiliation	Tianjin Medical University Eye Hospital
contact email	xiaorli@163.com
lab head
Manhong Xu
contact affiliation	Tianjin Medical University Eye Hospital
contact email	hollyxuu@gmail.com
dataset submitter

iProX dataset URI