PXD029207

PXD029207 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A BioID-derived proximity interactome for SARS-CoV-2 proteins
Description	The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here the cellular impact of expressing SARS-CoV-2 viral proteins was studied by global proteomic analysis and proximity biotinylation (BioID) was used to map the SARS-CoV-2 virus-host interactome in human lung cancer derived cells. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are associated with SARS-CoV-2 proteins. We have also established a website to host the proteomic data to allow for public access and continued analysis of host-viral protein associations and whole-cell proteomes of cells expressing the viral-BioID fusion proteins. Furthermore, we identified 154 high-confidence interactions by comparing this study with previous reports, providing a strong foundation for future follow-up studies. Finally, we cross-referenced candidate interactors with the CLUE drug library to identify potential therapeutics for drug-repurposing efforts. Collectively, these studies provide a valuable resource to uncover novel SARS-CoV-2 biology and inform development of antivirals.
HostingRepository	MassIVE
AnnounceDate	2022-11-29
AnnouncementXML	Submission_2022-11-29_23:39:31.667.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Rosa Campos
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 15:46:49	ID requested
⏵ 1	2022-11-29 23:39:32	announced

Publication List

May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL, Halfmann CT, D, í, az Pe, ñ, a R, Pratt D, Campos AR, Roux KJ, A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins. Viruses, 14(3):(2022) [pubmed]

May DG, Martin-Sancho L, Anschau V, Liu S, Chrisopulos RJ, Scott KL, Halfmann CT, D, í, az Pe, ñ, a R, Pratt D, Campos AR, Roux KJ, A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins. Viruses, 14(3):(2022) [pubmed]

Keyword List

submitter keyword: SARS-Cov2, BioID, protein-protein interaction, Shiny application

submitter keyword: SARS-Cov2, BioID, protein-protein interaction, Shiny application

Contact List

Alex Rosa Campos
contact affiliation	Sanford Burnham Prebys Medical Discovery Institute
contact email	arosacampos@sbpdiscovery.org
lab head
Alexandre Rosa Campos
contact affiliation	SBP Medical Discovery Institute
contact email	arosacampos@sbpdiscovery.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088245/