PXD029200

PXD029200 is an original dataset announced via ProteomeXchange.

Title	Label-free proteomic analysis of hepatocyte spheroids fed physiologically relevant levels of palmitic acid and oleic acid to mimic non-alcoholic fatty liver disease.
Description	We have grown hepatocyte spheroids for 21 days following an established protocol. We have carried out proteomic analysis of the difference between control and treated (Fatty acids) spheroids during seven days (5 timepoints at day 0, 1, 2, 4 and 7). Samples were digesting using filter aided sample preparation-based digestion and subsequently analysed using label free LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2021-11-24
AnnouncementXML	Submission_2021-11-24_04:30:01.944.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029200
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Adelina Rogowska-Wrzesinska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 04:50:57	ID requested
⏵ 1	2021-11-24 04:30:02	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, label-free, spheroids, liver, non-alcoholic fatty liver disease, NAFLD, hepatocyte, HepG2/C3A, rotary bioreactor, 3-dimensional cell culture

Adelina Rogowska-Wrzesinska
contact affiliation	University of southern Denmark Department of Biochemistry and Molecular Biology Protein Research group Denmark
contact email	Adelinar@bmb.sdu.dk
lab head
Adelina Rogowska-Wrzesinska
contact affiliation	University of Southern Denmark
contact email	adelinar@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD029200

PRIDE project URI

• PRIDE
  1. PXD029200
     1. Label: PRIDE project
     2. Name: Label-free proteomic analysis of hepatocyte spheroids fed physiologically relevant levels of palmitic acid and oleic acid to mimic non-alcoholic fatty liver disease.