PXD029181

PXD029181 is an original dataset announced via ProteomeXchange.

Title	Dysregulation of circulating protease activity in COVID-19-associated superinfection
Description	Infection by SARS-CoV-2 and subsequent COVID-19 can cause viral sepsis and septic shock. Our past studies demonstrated that dysregulated systemic proteolysis is associated with the pathological mechanism in bacterial septic shock. Thus, here we perform shotgun proteomics and peptidomics analysis by LC-MS/MS to identify and quantify the circulating protein and peptide profile of COVID-19 patient plasma. Plasma samples from four COVID-19 patients were collected at different time points of their ICU stay, including samples from a patient with COVID-19-induced sepsis and bacterial superinfection. By combining mass spectrometry analysis with enzymatic activity assays, our study elucidates the possible pathological involvement of proteolysis in COVID-19-induced sepsis, with particular insight into the dyregulation of protease-mediated systems, such as the coagulation cascade.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:57.071.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joyce Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 01:28:04	ID requested
1	2023-03-10 18:42:47	announced
⏵ 2	2023-11-14 08:29:57	announced	2023-11-14: Updated project metadata.

Dos Santos F, Li JB, Juocys N, Mazor R, Beretta L, Coufal NG, Lam MTY, Odish MF, Irigoyen MC, O'Donoghue AJ, Aletti F, Kistler EB, Plasma enzymatic activity, proteomics and peptidomics in COVID-19-induced sepsis: A novel approach for the analysis of hemostasis. Front Mol Biosci, 9():1051471(2022) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: peptidomics, proteomics,Covid-19, sepsis, proteolysis, enzymatic activity

Erik B. Kistler
contact affiliation	Department of Anesthesiology, School of Medicine, University of California, San Diego, United States Department of Anesthesiology & Critical Care, VA San Diego Healthcare System, United States
contact email	ekistler@health.ucsd.edu
lab head
Joyce Li
contact affiliation	UC San Diego
contact email	jbli@eng.ucsd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD029181

PRIDE project URI

• PRIDE
  1. PXD029181
     1. Label: PRIDE project
     2. Name: Dysregulation of circulating protease activity in COVID-19-associated superinfection