PXD029179

PXD029179 is an original dataset announced via ProteomeXchange.

Title	BaSCX method for enriching methylated peptieds
Description	Protein methylation as one of the most important post-translational modifications of proteins has been under the spotlight due to its essential role in many important biological processes. To data, antibody-based enrichment strategy is the most used approach to enrich methylated peptides. Unfortunately, its high cost prevents it from being widespread usage in most labs. In this paper, a cheap, friendly, and powerful method combined with basic strong cation exchange chromatography and multiple enzymes digestion was developed to achieve protein methylation identification. After enrichment, about 445 methylation forms on 376 methylation sites was identified from194 proteins in one single LC-MS/MS run using only 100 μg digests. And this method shows better performance for arginine methylation identification. Specially, the specificity of methylated peptides was up to 28.5%, this is the highest specificity towards methylated peptides as far as we known. In summary, this wonderful method has great potential in studying protein methylation, especially arginine methylation, regulated biological processes.
HostingRepository	iProX
AnnounceDate	2021-10-18
AnnouncementXML	Submission_2021-10-18_00:55:15.440.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhouxian Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	trimethylated residue; dimethylated residue; monomethylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 00:55:08	ID requested
⏵ 1	2021-10-18 00:55:16	announced

Li Z, Wang Q, Wang Y, Wang K, Liu Z, Zhang W, Ye M, An efficient approach based on basic strong cation exchange chromatography for enriching methylated peptides with high specificity for methylproteomics analysis. Anal Chim Acta, 1161():338467(2021) [pubmed]

submitter keyword: methlated peptides, basic strong cation exchange

Weibing Zhang
contact affiliation	East China University of Science and Technology
contact email	weibingzhang@ecust.edu.cn
lab head
Zhouxian Li
contact affiliation	East China University of Science and Technology
contact email	lizhouxian17@163.com
dataset submitter

iProX dataset URI