PXD029178

PXD029178 is an original dataset announced via ProteomeXchange.

Title	Selective enrichment of N-terminal proline peptides via hydrazide chemistry for proteomics analysis
Description	A challenge for shotgun proteomics is the identification of low abundance proteins, which is always hampered owing to the extreme complexity of protein digests and highly dynamic concentration range of proteins. To reduce the complexity of the peptide mixture, we developed a novel method to selectively enrich N-terminal proline peptides via hydrazide chemistry. This method consisted of ortho-phthalaldehyde (OPA) blocking of primary amines in peptides, reductive glutaraldehydation of N-terminal proline and solid phase hydrazide chemistry enrichment of aldehyde-modified N-terminal proline peptide.
HostingRepository	iProX
AnnounceDate	2021-10-18
AnnouncementXML	Submission_2021-10-18_00:53:08.540.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhouxian Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 00:52:53	ID requested
⏵ 1	2021-10-18 00:53:09	announced

Li Z, Wang Q, Mao J, Zhang L, Zhang W, Ye M, Selective enrichment of N-terminal proline peptides via hydrazide chemistry for proteomics analysis. Anal Chim Acta, 1142():48-55(2021) [pubmed]

submitter keyword: N-terminal proline peptidess, Lys-C/Arg-C digestion, solid-phase hydrazide chemistry

Weibing Zhang
contact affiliation	East China University of Science and Technology
contact email	weibingzhang@ecust.edu.cn
lab head
Zhouxian Li
contact affiliation	East China University of Science and Technology
contact email	lizhouxian17@163.com
dataset submitter

iProX dataset URI