PXD029167

PXD029167 is an original dataset announced via ProteomeXchange.

Title	Targeted mass spectrometry enables multiplexed quantification of immunomodulatory proteins in clinical biospecimens
Description	Immunotherapies are revolutionizing cancer care, producing durable responses and potentially cures in a subset of patients. However, response rates are low for most tumors, grade 3/4 toxicities are not uncommon, and our current understanding of tumor immunobiology is incomplete. While hundreds of immunomodulatory proteins in the tumor microenvironment shape the anti-tumor response, few of them can be reliably quantified. To address this need, we developed a multiplex panel of targeted proteomic assays to 52 peptides representing 46 proteins using peptide immunoaffinity enrichment coupled to multiple reaction monitoring-mass spectrometry. We validated the assays in tissue and plasma matrices, where performance figures of merit showed over 3 orders of dynamic range and median inter-day CVs of 5.2% (tissue) and 21% (plasma). A feasibility study in clinical biospecimens showed detection of 48/52 peptides in frozen tissue and 38/52 peptides in plasma. The assays are publicly available as a resource for the research community.
HostingRepository	PanoramaPublic
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_17:01:22.215.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeff Whiteaker
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Phospho; Label:13C(6); Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	QTRAP 5500

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-16 07:44:31	ID requested
1	2021-11-08 07:36:21	announced
⏵ 2	2022-02-28 17:01:23	announced	: Added PubMed Id

Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG, Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens. Front Immunol, 12():765898(2021) [pubmed]

submitter keyword: immuno-MRM, peptide immunoaffinity enrichment, immuno-oncology, immunotherapeutics, cancer

Jeff Whiteaker
contact affiliation	Fred Hutchinson Cancer Research Center
contact email	jwhiteak@fhcrc.org
lab head
Jeff Whiteaker
contact affiliation	Fred Hutchinson Cancer Research Center
contact email	jwhiteak@fhcrc.org
dataset submitter

Panorama Public dataset URI