PXD029164

PXD029164 is an original dataset announced via ProteomeXchange.

Title	Towards biorecycling of polyurethanes: Strategies of Pseudomonas capeferrum TDA1 for extra- and intracellular degradation
Description	The continuing reports of plastic pollution in various ecosystems highlight the threat posed by the ever-increasing consumption of synthetic polymers. Therefore, Pseudomonas capeferrum TDA1, a strain recently isolated from a plastic dump site, was examined further regarding its ability to degrade polyurethane (PU) compounds. The previously reported degradation pathway for 2,4-toluene diamine (2,4-TDA), a precursor and degradation intermediate of PU, could be confirmed by RNA-seq in this organism. In addition, different cell fractions of cells grown on a PU oligomer were tested for extracellular hydrolytic activity using a standard assay. Strikingly, purified outer membrane vesicles (OMV) of P. capeferrum TDA1 grown on a PU oligomer showed higher esterase activity than cell pellets. Hydrolases in the OMV fraction possibly involved in extracellular PU degradation were identified by mass spectrometry. On this basis, we propose a model for extracellular degradation of polyester-based PUs by P. capeferrum TDA1 involving the role of OMVs in synthetic polymer degradation.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:09:12.169.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nico Jehmlich
SpeciesList	scientific name: Pseudomonas sp. TDA1; NCBI TaxID: 2681304;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-15 06:58:55	ID requested
⏵ 1	2022-02-22 04:09:12	announced

Puiggen, é Ò, Espinosa MJC, Schlosser D, Thies S, Jehmlich N, Kappelmeyer U, Schreiber S, Wibberg D, Kalinowski J, Harms H, Heipieper HJ, Eberlein C, Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1. Sci Rep, 12(1):2666(2022) [pubmed]

submitter keyword: Proteomics, Outer membrane vesicles, Pseudomonas, polyurethane degradation

Nico Jehmlich
contact affiliation	Helmholtz Centre for Environmental Research GmbH - UFZ Department of Molecular Systems Biology Permoserstraße 15, 04318 Leipzig, Germany
contact email	nico.jehmlich@ufz.de
lab head
Nico Jehmlich
contact affiliation	Helmholtz-Centre for Environmental Research - UFZ
contact email	nico.jehmlich@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029164
     1. Label: PRIDE project
     2. Name: Towards biorecycling of polyurethanes: Strategies of Pseudomonas capeferrum TDA1 for extra- and intracellular degradation