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PXD029146

PXD029146 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman Cytomegalovirus modifies placental small extracellular vesicle secretion and composition to enhance infection of fetal recipient cells
DescriptionHuman cytomegalovirus (hCMV) primo-infection, reinfection and/or reactivation is a major issue during pregnancy and affects 1% of live births in western countries, making hCMV the most frequently transmitted virus in utero. Despite the extensive research conducted so far, the pathophysiology of this congenital infection remains unclear. Recently, increasing evidence point out the role of small extracellular vesicles (sEVs) in cell-cell communication underlying the feto-placenta-maternal dialogue during pregnancy. In this study, we examined the impact of hCMV infection on the protein composition and function of placental sEVs. We observed that infection of placental cells led to an alteration of protein composition of their secreted sEVs, suggesting that placental sEVs may acquire a proviral phenotype. Functional studies performed on fetal recipient cells, notably neural stem cells, confirmed the ability of sEVs produced by infected cells to facilitate further infection of naive recipient cells. Altogether, our study demonstrates that placental sEVs are key players of hCMV pathophysiology during congenital infection, and may favor the transmission of the virus towards the fetus.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:08:33.887.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarlene Marcellin
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-15 03:22:34ID requested
12022-10-13 08:53:58announced
22023-11-14 08:08:34announced2023-11-14: Updated project metadata.
Publication List
Bergamelli M, Martin H, Aubert Y, Mansuy JM, Marcellin M, Burlet-Schiltz O, Hurbain I, Raposo G, Izopet J, Fournier T, Benchoua A, B, é, nard M, Groussolles M, Cartron G, Tanguy Le Gac Y, Moinard N, D'Angelo G, Malnou CE, Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro. Viruses, 14(9):(2022) [pubmed]
Keyword List
submitter keyword: neural stem cells,hCMV, congenital infection, extracellular vesicles, placenta, cytotrophoblast
Contact List
Odile Burlet-Schiltz
contact affiliationInstitut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France.
contact emailOdile.Schiltz@ipbs.fr
lab head
Marlene Marcellin
contact affiliationCNRS
contact emailmarlene.marcellin@ipbs.fr
dataset submitter
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Dataset FTP location
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