PXD029134

PXD029134 is an original dataset announced via ProteomeXchange.

Title	Melatonin promotes chilling tolerance of cucumber seedlings by regulating antioxidant systems and relieving photoinhibition
Description	Chilling is a major stress which adversely decreases photosynthesis and leads to oxidative stress in thermophilic plants. The role of melatonin (MT) in stress response of plants, has so far been investigated, while the mechanisms by which MT regulate photosynthesis in chilling-sensitive cucumber under chilling stress remain unclear. In this paper, we reported MT positively regulated chilling tolerance of cucumber. Moreover, MT showed a concentration-dependent manner and 1.0 μmol·L-1 was the optimum concentration, of which the chilling injury index, EL and MDA were the lowest, while growth was the highest among all the treatments. Further studies indicated that MT triggered the activity and expression of the antioxidant enzymes, which in turn decreased ROS accumulation caused by chilling stress. Meanwhile, MT maintained a high photosynthetic carbon assimilation capacity, increased the activity of PSI reaction center, PSII reaction center and electron transfer efficiency. More importantly, the proteome analysis and western-blot data revealed that PSI-associated proteins (PsaD, PsaE, PsaF, PsaH and PsaN), PSII-associated protein D1 as well as Rubisco large subunit and RCA were significantly up-regulated in MT-treated seedlings, compared to H2O-treated seedlings under chilling stress. These results suggest that MT enhances chilling tolerance of cucumber via activating the antioxidant system as well as protein level of key PSI-, PSII-related and carbon assimilation enzymes to decrease ROS accumulation, and finally alleviates the damage to photosynthetic apparatus under chilling stress.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_11:42:37.680.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaowei Zhang
SpeciesList	scientific name: Cucumis sativus; NCBI TaxID: 3659;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-14 08:00:01	ID requested
⏵ 1	2022-02-15 11:42:38	announced

Zhang X, Feng Y, Jing T, Liu X, Ai X, Bi H, Melatonin Promotes the Chilling Tolerance of Cucumber Seedlings by Regulating Antioxidant System and Relieving Photoinhibition. Front Plant Sci, 12():789617(2021) [pubmed]

submitter keyword: Melatonin, Reactive oxygen, Antioxidant system, Photosynthesis, Chilling stress, Cucumber

Huan gai Bi
contact affiliation	Shandong Agricultural University
contact email	bhg163@163.com
lab head
Xiaowei Zhang
contact affiliation	Shandong Agricultural University
contact email	2019010077@sdau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029134

PRIDE project URI

• PRIDE
  1. PXD029134
     1. Label: PRIDE project
     2. Name: Melatonin promotes chilling tolerance of cucumber seedlings by regulating antioxidant systems and relieving photoinhibition