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PXD029122 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleModular bottom-up proteomic sample preparation for high-throughput applications
DescriptionA collection of digital automated proteomic sample preparation protocols detail three optimized step-by-step methods to: (A) lyse Gram-negative bacteria and fungal cells; (B) quantify the amount of protein extracted; and (C) normalize the amount of protein and set up tryptic digestion. These protocols have been developed to facilitate rapid, low variance sample preparation of hundreds of samples, be easily implemented on widely-available Beckman-Coulter Biomek automated liquid handlers, and allow flexibility for future protocol development
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Petzold
SpeciesList scientific name: Pseudomonas putida; NCBI TaxID: 303; scientific name: Rhodotorula toruloides; NCBI TaxID: 5286;
ModificationListdeaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-13 23:06:47ID requested
12022-02-14 13:24:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proteomic sample preparation, automation, high-throughput
Contact List
Christopher J. Petzold
contact affiliationStaff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact emailcjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliationLawrence Berkeley National Laboratory
contact emailcjpetzold@lbl.gov
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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