PXD029119

PXD029119 is an original dataset announced via ProteomeXchange.

Title	Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW
Description	In addition to their well-known role as stress-associated catecholamine hormones in animals and humans, epinephrine (EPI) and norepinephrine (NE) act as interkingdom signals between eukaryotic hosts and bacteria. However, the molecular basis of their effects on bacteria is not well understood. In initial phenotypic studies utilizing Vibrio campbellii as a model organism, we characterized the bipartite mode of action of catecholamines, which consists of promotion of growth under iron limitation, and enhanced colony expansion. In order to identify the molecular targets of the hormones, we designed and synthesized tailored probes for chemical proteomic studies. As the catechol group in EPI and NE acts as iron chelator and is prone to form a reactive quinone moiety, we devised a photoprobe based on the adrenergic agonist phenylephrine (PE), which solely influenced colony expansion on soft agar. Using this probe, we identified CheW, located at the core of the chemotaxis signaling network, as a major target. In vitro studies confirmed that EPI, NE, PE, as well as labetalol, a clinically applied antagonist, bind to purified CheW with affinity constants in the sub-micromolar range. In line with these findings, exposure of V. campbellii to these adrenergic agonists affects the chemotactic control of the bacterium. This study highlights a previously unknown effect of eukaryotic signaling molecules on bacterial motility.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_06:41:06.652.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angela Weigert Muñoz
SpeciesList	scientific name: Vibrio campbellii ATCC BAA-1116; NCBI TaxID: 338187;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-13 23:02:39	ID requested
⏵ 1	2022-05-31 06:41:07	announced

Weigert Mu, ñ, oz A, Hoyer E, Schumacher K, Grognot M, Taute KM, Hacker SM, Sieber SA, Jung K, by binding to the coupling protein CheW. Proc Natl Acad Sci U S A, 119(10):e2118227119(2022) [pubmed]

submitter keyword: V. campbellii, Q Exactive Plus, Orbitrap Fusion, Epinephrine, Phenylephrine

Stephan Axel Sieber
contact affiliation	Center for Functional Protein Assemblies, Technical University of Munich, Germany
contact email	stephan.sieber@tum.de
lab head
Angela Weigert Muñoz
contact affiliation	Technical University of Munich
contact email	angela.weigert@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029119
     1. Label: PRIDE project
     2. Name: Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW