PXD029101

PXD029101 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Dictyostelium discoideum mitochondria
Description	There is a paucity of data regarding what proteins localize to the mitochondria in the social amoeba Dictyostelium discoideum. Therefore, here, we utilized high-throughput tandem mass tag (TMT)-based protein quantitation to identify proteins in the whole-cell lysate, as well as in crude and purified mitochondrial samples. We then calculated the ratio of a protein’s abundance in the mitochondria isolates, both crude and highly purified, versus its abundance in the whole-cell lysate. The resulting value, indicating its enrichment in mitochondrial preparations, was further normalized to the average enrichment ratio of 47 reference mitochondrial proteins, to compute the relative enrichment ratio. Using this approach, we identified 908 putative mitochondrial proteins. We ultimately combined proteomic and in silico analyses to yield a comprehensive mitochondrial protein compendium. Our compendium lays the foundation for future studies to understand the functions of conserved mitochondrial proteins in health and diseases using D. discoideum as the model. It also provides an entry to study many fascinating mitochondrial processes that are unique in protists. Additionally, through comparative genomics, our compendium will complement mitochondrial protein discovery in other organisms and may shed light on the evolution of mitochondrial proteomes and processes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:09.719.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029101
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yong Chen
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-12 23:34:31	ID requested
1	2022-06-06 10:39:02	announced
⏵ 2	2023-11-14 08:52:10	announced	2023-11-14: Updated project metadata.

10.6019/PXD029101;

submitter keyword: Dictyostelium discoideum, proteomics, TMT, mitochondria purification, LCMSMS

Hong Xu
contact affiliation	The Laboratory of Molecular Genetics, National Heart Lung and Blood Institute
contact email	xuh5@nhlbi.nih.gov
lab head
Yong Chen
contact affiliation	NIH
contact email	yong.chen@nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029101
     1. Label: PRIDE project
     2. Name: Proteomics of Dictyostelium discoideum mitochondria