PXD029091

PXD029091 is an original dataset announced via ProteomeXchange.

Title	A genetically-encoded crosslinker screen identifies SERBP1 as a PKC substrate influencing translation and cell division
Description	The PKC-regulated genome protective pathway provides transformed cells a failsafe to successfully complete mitosis. Despite the necessary role for Aurora B in this programme, it is unclear whether its requirement is sufficient or if other PKC cell cycle targets are involved. To address this, we developed a trapping strategy using UV-photocrosslinkable amino acids encoded in the PKC kinase domain. The validation of the mRNA binding protein SERBP1 as a PKC substrate revealed a series of mitotic events controlled by the catalytic form of PKC. PKC represses protein translation, altering SERBP1 binding to the 40S ribosomal subunit and promoting the assembly of ribonucleoprotein granules containing SERBP1, termed M-bodies. Independent of Aurora B, SERBP1 is shown to be necessary for chromosome segregation and successful cell division, correlating with M-body formation. This requirement for SERBP1 demonstrates that Aurora B acts in concert with translational regulation in the PKC-controlled pathway exerting genome protection.
HostingRepository	PRIDE
AnnounceDate	2023-04-11
AnnouncementXML	Submission_2023-04-11_06:25:12.306.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AndrewJones
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-12 08:18:36	ID requested
1	2021-12-09 03:29:25	announced
⏵ 2	2023-04-11 06:25:15	announced	2023-04-11: Updated project metadata.

Martini S, Davis K, Faraway R, Elze L, Lockwood N, Jones A, Xie X, McDonald NQ, Mann DJ, Armstrong A, Ule J, Parker PJ, substrate influencing translation and cell division. Nat Commun, 12(1):6934(2021) [pubmed]

submitter keyword: Human

PeterParker
contact affiliation	Protein Phosphorylation Laboratory, The Francis Crick Institute
contact email	Peter.Parker@crick.ac.uk
lab head
AndrewJones
contact affiliation	The Francis Crick Institute
contact email	Andrew.Jones@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD029091

PRIDE project URI

• PRIDE
  1. PXD029091
     1. Label: PRIDE project
     2. Name: A genetically-encoded crosslinker screen identifies SERBP1 as a PKC substrate influencing translation and cell division