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PXD029084

PXD029084 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSearching for interactors of endogenous SLX4
DescriptionThe project was aimed at identifying binding partners of endogenous SLX4 in HeLa cells. Whole cell extracts from HeLa Flp-In T-Rex cells were used for immunoprecipitation performed with anti-SLX4 antibodies from Bethyl Laboratories (A302-270A and A302-269A), either used alone or in combination. To discriminate bona fide SLX4 partners from non-specific proteins recognized by the anti-SLX4 antibodies, cells were transfected with control (Luc) siRNA or a siRNA targeting SLX4. After immunoprecipitation, protein complexes were analysed by LC-MSMS.
HostingRepositoryPRIDE
AnnounceDate2022-02-16
AnnouncementXMLSubmission_2022-02-16_03:36:59.243.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAUDEBERT Stephane
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; mono N-acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-12 08:08:36ID requested
12022-02-16 03:37:00announced
Publication List
Guervilly JH, Blin M, Laureti L, Baudelet E, Audebert S, Gaillard PH, -dependent mismatch repair. Nucleic Acids Res, 50(5):2667-2680(2022) [pubmed]
Keyword List
submitter keyword: LC-MSMS, endogenous SLX4,Interactome
Contact List
Pierre-Henri Gaillard
contact affiliationCRCM, Institut Paoli-Calmettes, Aix-Marseille Université, Inserm, CNRS, 13009 Marseille, France
contact emailpierre-henri.gaillard@inserm.fr
lab head
AUDEBERT Stephane
contact affiliationMarseille Proteomic, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes, 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France
contact emailstephane.audebert@inserm.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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