PXD029070 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Rat vascular smooth muscle cells LC-MS/MS |
Description | Crotonylation of histones is discovered of late as one of post-translational modification that can regulate gene expression. However, the function of crotonylation on non-histone proteins in vascular smooth muscle cells (VSMC) is unclear. Here, we aim to use modification and proteomic analysis to find the cellular characteristic of crotonylated non-histone proteins and the crosstalk with ubiquitinated proteins in vascular smooth muscle cell (VSMC) phenotypic remodeling. We performed modification and proteomic analysis of VSMCs before and after stimulated with platelet-derived growth factor-BB (PDGF-BB). The crotonylated and ubiquitinated pan-antibody was used to enrich the protein and then subjected to high-throughput mass spectrometry analysis. The enrichment analysis was performed within differentially modified proteins in regards to GO terms, KEGG and protein domain. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-19 |
AnnouncementXML | Submission_2022-05-19_03:43:10.087.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Li-Hua Dong |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | ubiquitinylated lysine; crotonylated L-lysine |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-11 22:11:10 | ID requested | |
⏵ 1 | 2022-05-19 03:43:11 | announced | |
Publication List
Cao SH, Chen ZH, Ma RY, Yue L, Jiang HM, Dong LH, Dynamics and Functional Interplay of Nonhistone Lysine Crotonylome and Ubiquitylome in Vascular Smooth Muscle Cell Phenotypic Remodeling. Front Cardiovasc Med, 9():783739(2022) [pubmed] |
Keyword List
submitter keyword: VSMC, LC-MS/MS |
Contact List
Li-Hua Dong |
contact affiliation | Department of Biochemistry and Molecular Biology, College of Basic Medicine, Key Laboratory of Medical Biotechnology of Hebei Province, Hebei Medical University, Shijiazhuang, China |
contact email | donglihua@hebmu.edu.cn |
lab head | |
Li-Hua Dong |
contact affiliation | Hebei Medical University |
contact email | donglihua@hebmu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029070
- Label: PRIDE project
- Name: Rat vascular smooth muscle cells LC-MS/MS