PXD029062 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identifying the N-terminus of SLX4Nter in HeLa KO30 cells |
Description | The project was aimed at identifying the N-terminus of a truncated form of SLX4 (termed SLX4Nter) in HeLa KO30 cells. The HeLa KO30 cell line comes from a clone of HeLa Flp-In T-Rex cells (termed FITo) obtained through a CRISPR-Cas9 approach using commercially available plasmids from Santa Cruz Biotechnology (sc-404395 and sc-404395-HDR). This allows the integration of an exogenous plasmid conferring Puromycin resistance at the endogenous SLX4 locus. Unexpectedly, this strategy allowed us to retrieve several Puromycin-resistant clones (including KO30) displaying a shorter form of endogenous SLX4 that is N-terminally truncated (SLX4Nter). To identify the proximal peptide of SLX4Nter, FITo and KO30 nuclear extracts were immunoprecipitated with anti-SLX4 antibodies and N-terminus of SLX4Nter was further identified by LC-MSMS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_03:32:14.907.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AUDEBERT Stephane |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; mono N-acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-11 09:02:23 | ID requested | |
⏵ 1 | 2022-02-16 03:32:15 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SLX4, CRISPR-Cas9, N-terminal truncation, N-terminus acetylation |
Contact List
Pierre-Henri Gaillard |
contact affiliation | CRCM, Institut Paoli-Calmettes, Aix-Marseille Université, Inserm, CNRS, 13009 Marseille, France |
contact email | pierre-henri.gaillard@inserm.fr |
lab head | |
AUDEBERT Stephane |
contact affiliation | Marseille Proteomic, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes, 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France |
contact email | stephane.audebert@inserm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029062
- Label: PRIDE project
- Name: Identifying the N-terminus of SLX4Nter in HeLa KO30 cells