PXD029057

PXD029057 is an original dataset announced via ProteomeXchange.

Title	ChIRP-MS of long non-coding RNA HOTAIR
Description	Accumulating evidence highlights the role of long non-coding RNAs (lncRNA) in cellular homeostasis, and their dysregulation in disease settings. Most lncRNAs function by interacting with proteins or protein complexes. While several orthogonal methods have been developed to identify these proteins, each method has its inherent strengths and limitations. Here, we combine two RNA-centric methods ChIRP-MS and RNA-BioID to obtain a comprehensive list of proteins that interact with the well-known lncRNA HOTAIR. Overexpression of HOTAIR has been associated with a metastasis-promoting phenotype in various cancers. Although HOTAIR is known to bind with PRC2 and LSD1 protein complexes, an unbiased and comprehensive method to map its interactome has not yet been performed. Both ChIRP-MS and RNA-BioID data sets show an association of HOTAIR with mitoribosomes, suggesting HOTAIR has functions independent of its (post-)transcriptional mode-of-action.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:25:11.441.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louis Delhaye
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-11 03:56:15	ID requested
⏵ 1	2022-02-15 09:25:12	announced

Delhaye L, De Bruycker E, Volders PJ, Fijalkowska D, De Sutter D, Degroeve S, Martens L, Mestdagh P, Eyckerman S, Orthogonal proteomics methods to unravel the HOTAIR interactome. Sci Rep, 12(1):1513(2022) [pubmed]

submitter keyword: lncRNA, ChIRP-MS, HOTAIR

Sven Eyckerman
contact affiliation	VIB-UGent Center for Medical Biotechnology
contact email	sven.eyckerman@ugent.vib.be
lab head
Louis Delhaye
contact affiliation	VIB-UGent
contact email	louis.delhaye@ugent.vib.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029057

PRIDE project URI

• PRIDE
  1. PXD029057
     1. Label: PRIDE project
     2. Name: ChIRP-MS of long non-coding RNA HOTAIR