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PXD029047

PXD029047 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMultilayered Regulation of Autophagy by the Atg1 Kinase Orchestrates Spatial and Temporal Control of Autophagosome Formation
DescriptionAutophagy is a conserved intracellular degradation pathway exerting various cytoprotective and homeostatic functions by utilizing de novo double membrane vesicle (autophagosome) formation to target a wide range of cytoplasmic material for vacuolar/lysosomal degradation. The Atg1 kinase is one of its key regulators coordinating a complex signaling program to orchestrate autophagosome formation. Combining in vitro reconstitution and cell based approaches, we demonstrate that Atg1 is activated by lipidated Atg8 (Atg8-PE) stimulating substrate phosphorylation along the growing autophagosomal membrane. Atg1 dependent phosphorylation of Atg13 triggers Atg1 complex dissociation resulting in rapid turnover of Atg1 complex subunits at the pre-autophagosomal structure. Moreover, Atg1 recruitment by Atg8-PE self-regulates Atg8-PE levels in the growing autophagosomal membrane by phosphorylating and inhibiting the Atg8 specific E2 and E3. Taken together, our work uncovers the molecular basis for positive and negative feedback imposed by Atg1, and opposing phosphorylation and dephosphorylation events governing the spatiotemporal regulation of autophagy.
HostingRepositoryPRIDE
AnnounceDate2021-10-11
AnnouncementXMLSubmission_2021-10-11_13:57:39.113.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBen Collins
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue
InstrumenttimsTOF Pro; TripleTOF 5600; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-11 02:17:18ID requested
12021-10-11 13:57:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Autophagy, signalling, phosphorylation, protein kinases, protein phosphatases, ubiquitin-like proteins, Atg8 lipidation, metabolism
Contact List
Ben Collins
contact affiliationQueen's University Belfast
contact emailben.collins@qub.ac.uk
lab head
Ben Collins
contact affiliationQueen's University Belfast
contact emailben.collins@qub.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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