PXD029015

PXD029015 is an original dataset announced via ProteomeXchange.

Title	Human serum extracellular vesicle proteomic profile depends on the enrichment method employed
Description	The proteomic profiling of serum samples supposes a challenge due to the large abundance of few blood proteins in comparison with other circulating proteins coming from different tissues and cells. Although the sensitivity of protein detection has increased enormously in the last years, to enrich less abundant proteins, getting rid of abundant proteins such as albumin, lipoproteins, and immunoglobulins, require specific strategies. One of the alternatives that has become more promising is to characterize circulating extracellular vesicles from serum samples. In the present work, we enriched the extracellular vesicles fraction from human serum by applying different techniques, including ultracentrifugation, size exclusion chromatography, and two commercial precipitation methods based on different mechanism of action. To improve the performance of the techniques and promote purity of the preparations we have employed samples of 80 ul. The comparative proteomic profiling of the enriched preparations shows that ultracentrifugation procedure yields a larger and completely different set of proteins than other techniques, including mitochondrial and ribosome related proteins. Size exclusion chromatography carries over lipoprotein associated proteins, while a polymer-based precipitation kit had more affinity for proteins associated with granules of platelets. The precipitation kit that targets glycosylation molecules enriched differentially protein harboring glycosylation sites, including immunoglobulins and proteins of the membrane attack complex.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:31:28.513.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mikel Azkargorta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-08 06:51:21	ID requested
⏵ 1	2022-02-17 00:31:29	announced

Azkargorta M, Iloro I, Escobes I, Cabrera D, Falcon-Perez JM, Elortza F, Royo F, Human Serum Extracellular Vesicle Proteomic Profile Depends on the Enrichment Method Employed. Int J Mol Sci, 22(20):(2021) [pubmed]

submitter keyword: extracellular vesicles, ultracentrifugation, size exclusion, precipitation kits, proteomic, serum

Felix Elortza
contact affiliation	Proteomics Platform CIC bioGUNE Bizkaia Tech. Park Build. 800 48160 Derio (Spain)
contact email	felortza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliation	Proteomics Platform CIC bioGUNE
contact email	mazkargorta@cicbiogune.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029015

PRIDE project URI

• PRIDE
  1. PXD029015
     1. Label: PRIDE project
     2. Name: Human serum extracellular vesicle proteomic profile depends on the enrichment method employed