PXD029007

PXD029007 is an original dataset announced via ProteomeXchange.

Title	Plasma-derived Exosome-Like Vesicles (ELVs) of COVID-19 patients expose SARS-CoV-2-Spike-derived fragments and contribute to the adaptive immune response
Description	COVID-19 is an infectious disease caused by beta-coronavirus SARS-CoV-2 which has rapidly spread across the globe starting from February 2020. It is well established that during viral infection extracellular vesicles become delivery/presenting vectors of viral material. However, studies regarding extracellular vesicle function in COVID-19 pathology are still scanty. Here, we performed a comparative study on plasma-derived Exosome-Like Vesicles (ELVs) of either MILD or SEVERE COVID-19 patients. We show that, although both types of vesicles efficiently display SARS-CoV-2-Spike-derived peptides and carry immunomodulatory molecules, only those of MILD patients are capable to efficiently regulate antigen-specific CD4+ T cell responses. Accordingly, by mass spectrometry we show that the proteome of ELVs of MILD patients correlates with a proper functioning of the immune system, while that of SEVERE patients is associated with increased and chronic inflammation. Overall, we show, that plasma-derived ELVs of COVID-19 patients possess SARS-CoV-2-derived protein material, have an active role in enhancing the immune response and possess a cargo which reflects the pathological state of patients in the acute phase of the disease.
HostingRepository	PRIDE
AnnounceDate	2022-02-11
AnnouncementXML	Submission_2022-02-11_08:54:41.300.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	alessandro cuomo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-08 06:42:02	ID requested
1	2022-02-11 07:13:26	announced
⏵ 2	2022-02-11 08:54:42	announced	2022-02-11: Updated publication reference for PubMed record(s): 35111156.

Pesce E, Manfrini N, Cordiglieri C, Santi S, Bandera A, Gobbini A, Gruarin P, Favalli A, Bombaci M, Cuomo A, Collino F, Cricr, ì G, Ungaro R, Lombardi A, Mangioni D, Muscatello A, Aliberti S, Blasi F, Gori A, Abrignani S, De Francesco R, Biffo S, Grifantini R, Exosomes Recovered From the Plasma of COVID-19 Patients Expose SARS-CoV-2 Spike-Derived Fragments and Contribute to the Adaptive Immune Response. Front Immunol, 12():785941(2021) [pubmed]

submitter keyword: Exosome-Like Vesicles (ELVs), LCMSMS, COVID-19

Renata M. Grifantini
contact affiliation	National Institute of Molecular Genetics (INGM), Italy
contact email	grifantini@ingm.org
lab head
alessandro cuomo
contact affiliation	IEO
contact email	alessandro.cuomo@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029007

PRIDE project URI

• PRIDE
  1. PXD029007
     1. Label: PRIDE project
     2. Name: Plasma-derived Exosome-Like Vesicles (ELVs) of COVID-19 patients expose SARS-CoV-2-Spike-derived fragments and contribute to the adaptive immune response