PXD028992

PXD028992 is an original dataset announced via ProteomeXchange.

Title	Hexokinase 2 is a transcriptional targetHexokinase 2 is a transcriptional target and a positive modulator of AHR signalling and a positive modulator of AHR signalling
Description	The Aryl Hydrocarbon Receptor (AHR) regulates the expression of numerous genes in response to activation by agonists including xenobiotics. Although it is well appreciated that environmental signals and cell intrinsic features may modulate this transcriptional response, how it is mechanistically achieved remains poorly understood. We show that Hexokinase 2 (HK2) a metabolic enzyme fuelling cancer cell growth, is a transcriptional target of AHR as well as a modulator of its activity. Expression of HK2 is positively regulated by AHR upon exposure to agonists both in human cells and in mice lung tissues. Conversely, over-expression of HK2 regulates the abundance of many proteins involved in the regulation of AHR signalling and these changes are linked with altered AHR expression levels and transcriptional activity. HK2 expression also shows a negative correlation with AHR promoter methylation in tumours, and these tumours with high HK2 expression and low AHR methylation are associated with a worse overall survival in patients. In sum, our study provides novel insights into how AHR signalling is regulated which may help our understanding of the context-specific effects of this pathway and may have implications in cancer.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_01:47:26.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benoit Miotto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-07 08:57:56	ID requested
⏵ 1	2022-05-31 01:47:26	announced

Watzky M, Huard S, Juricek L, Dairou J, Chauvet C, Coumoul X, Letessier A, Miotto B, Hexokinase 2 is a transcriptional target and a positive modulator of AHR signalling. Nucleic Acids Res, 50(10):5545-5564(2022) [pubmed]

submitter keyword: Human

IOsteosarcoma

Hexokinase 2

AHR

Benoit Miotto
contact affiliation	INSERM U1016, Institut Cochin, Faculté de Médecine, Paris, FRANCE
contact email	benoit.miotto@inserm.fr
lab head
Benoit Miotto
contact affiliation	INSERM U1016
contact email	benoit.miotto@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028992
     1. Label: PRIDE project
     2. Name: Hexokinase 2 is a transcriptional targetHexokinase 2 is a transcriptional target and a positive modulator of AHR signalling and a positive modulator of AHR signalling