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PXD028991

PXD028991 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNew insights on the role of HCN in root hair elongation through single cell proteomics.
DescriptionIn order to identify the possible protein targets of cyanide in the regulation of root hair, we have carried out a single cell proteomic approach to characterize the protein atlas in wild type root hair cells compared to the cas-c1 mutant cells. Root hair specific cells were obtained from isolated protoplast from root tissues of Arabidopsis wt-pCOBL9:GFP and cas-c1-pCOBL9:GFP lines. To analyze the effect of the CAS-C1 mutation, we isolated 1 x 106 root hair cells by Fluorescence-activated cell sorting (FACS) from three independent replicate of wt-pCOBL9:GFP and cas-c1-pCOBL9:GFP roots. The extracted proteins from each sample were trypsin-digested and the digested peptides were analyzed by liquid chromatography-high resolution mass spectrometry (LC-MS/MS) for protein identification. In wild type samples, we have identified 3829 unique proteins at a false discovery rate below 1% (FDR<1%), that represent almost 10% of the total Arabidopsis proteome. In protein extract of the cas-c1-pCOBL9:GFP roots samples, we have identified 3972 proteins of which 3515 were commons in both cell types, 310 were only identified y wild type and 457 only in cas-c1 mutant (Fig. 5).
HostingRepositoryPRIDE
AnnounceDate2024-01-26
AnnouncementXMLSubmission_2024-01-26_06:07:53.671.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuis C. Romero
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmethylthiolated residue; monohydroxylated residue; iodoacetic acid derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-07 08:57:55ID requested
12024-01-26 06:07:54announced
Publication List
Arenas-Alfonseca L, Yamada M, Romero LC, Garc, í, a I, -Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics. Plants (Basel), 12(23):(2023) [pubmed]
10.3390/plants12234055;
Keyword List
submitter keyword: cyanoalanine, cyanide,plant, cyanylation
Contact List
Luis C. Romero
contact affiliationInstituto de Bioquímca Vegetal y Fotosíntesis-CSIC Avda. Américo Vespucio, 49 41092-Sevilla Spain
contact emaillromero@ibvf.csic.es
lab head
Luis C. Romero
contact affiliationInstituto Bioquimica Vegetal y Fotosintesis-CSIC
contact emaillromero@ibvf.csic.es
dataset submitter
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