PXD028991

PXD028991 is an original dataset announced via ProteomeXchange.

Title	New insights on the role of HCN in root hair elongation through single cell proteomics.
Description	In order to identify the possible protein targets of cyanide in the regulation of root hair, we have carried out a single cell proteomic approach to characterize the protein atlas in wild type root hair cells compared to the cas-c1 mutant cells. Root hair specific cells were obtained from isolated protoplast from root tissues of Arabidopsis wt-pCOBL9:GFP and cas-c1-pCOBL9:GFP lines. To analyze the effect of the CAS-C1 mutation, we isolated 1 x 106 root hair cells by Fluorescence-activated cell sorting (FACS) from three independent replicate of wt-pCOBL9:GFP and cas-c1-pCOBL9:GFP roots. The extracted proteins from each sample were trypsin-digested and the digested peptides were analyzed by liquid chromatography-high resolution mass spectrometry (LC-MS/MS) for protein identification. In wild type samples, we have identified 3829 unique proteins at a false discovery rate below 1% (FDR<1%), that represent almost 10% of the total Arabidopsis proteome. In protein extract of the cas-c1-pCOBL9:GFP roots samples, we have identified 3972 proteins of which 3515 were commons in both cell types, 310 were only identified y wild type and 457 only in cas-c1 mutant (Fig. 5).
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:07:53.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luis C. Romero
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	methylthiolated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-07 08:57:55	ID requested
⏵ 1	2024-01-26 06:07:54	announced

Arenas-Alfonseca L, Yamada M, Romero LC, Garc, í, a I, -Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics. Plants (Basel), 12(23):(2023) [pubmed]

10.3390/plants12234055;

submitter keyword: cyanoalanine, cyanide,plant, cyanylation

Luis C. Romero
contact affiliation	Instituto de Bioquímca Vegetal y Fotosíntesis-CSIC Avda. Américo Vespucio, 49 41092-Sevilla Spain
contact email	lromero@ibvf.csic.es
lab head
Luis C. Romero
contact affiliation	Instituto Bioquimica Vegetal y Fotosintesis-CSIC
contact email	lromero@ibvf.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028991
     1. Label: PRIDE project
     2. Name: New insights on the role of HCN in root hair elongation through single cell proteomics.