PXD028991 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | New insights on the role of HCN in root hair elongation through single cell proteomics. |
Description | In order to identify the possible protein targets of cyanide in the regulation of root hair, we have carried out a single cell proteomic approach to characterize the protein atlas in wild type root hair cells compared to the cas-c1 mutant cells. Root hair specific cells were obtained from isolated protoplast from root tissues of Arabidopsis wt-pCOBL9:GFP and cas-c1-pCOBL9:GFP lines. To analyze the effect of the CAS-C1 mutation, we isolated 1 x 106 root hair cells by Fluorescence-activated cell sorting (FACS) from three independent replicate of wt-pCOBL9:GFP and cas-c1-pCOBL9:GFP roots. The extracted proteins from each sample were trypsin-digested and the digested peptides were analyzed by liquid chromatography-high resolution mass spectrometry (LC-MS/MS) for protein identification. In wild type samples, we have identified 3829 unique proteins at a false discovery rate below 1% (FDR<1%), that represent almost 10% of the total Arabidopsis proteome. In protein extract of the cas-c1-pCOBL9:GFP roots samples, we have identified 3972 proteins of which 3515 were commons in both cell types, 310 were only identified y wild type and 457 only in cas-c1 mutant (Fig. 5). |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_06:07:53.671.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luis C. Romero |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | methylthiolated residue; monohydroxylated residue; iodoacetic acid derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-07 08:57:55 | ID requested | |
⏵ 1 | 2024-01-26 06:07:54 | announced | |
Publication List
Arenas-Alfonseca L, Yamada M, Romero LC, Garc, í, a I, -Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics. Plants (Basel), 12(23):(2023) [pubmed] |
10.3390/plants12234055; |
Keyword List
submitter keyword: cyanoalanine, cyanide,plant, cyanylation |
Contact List
Luis C. Romero |
contact affiliation | Instituto de Bioquímca Vegetal y Fotosíntesis-CSIC Avda. Américo Vespucio, 49 41092-Sevilla Spain |
contact email | lromero@ibvf.csic.es |
lab head | |
Luis C. Romero |
contact affiliation | Instituto Bioquimica Vegetal y Fotosintesis-CSIC |
contact email | lromero@ibvf.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028991
- Label: PRIDE project
- Name: New insights on the role of HCN in root hair elongation through single cell proteomics.