PXD028980

PXD028980 is an original dataset announced via ProteomeXchange.

Title	Proximity Proteomics Revealed Dicer1 and Transporters as Client Proteins of Aha1
Description	Co-chaperone Aha1 activates HSP90 ATPase to promote the folding of client proteins. However, the client proteins of Aha1 are largely unknown. By employing ascorbate peroxidase (APEX) based proximity labeling, we identified 32 proximity proteins of HSP90 that are modulated by genetic depletion of Aha1. Among them, Dicer1 is one of the top-ranked proteins, which were further confirmed by streptavidin pull-down followed by Western blot analysis, demonstrating the reliability of the approach. Flag pull-down result showed interactions between endogenous HSP90 and Dicer1 and Aha1. The Dicer1 level is regulated synergistically by Aha1 and HSP90. Maturation-dependent interaction results showed a preferential binding of Aha1 and HSP90 to nascently translated Dicer1. Reconstitution of Aha1-depleted cells with WT Aha1 restored Dicer1 level, while the HSP90-binding-defective E67K mutant exhibited partial restoration. Moreover, knockdown of Aha1 and inhibition of HSP90 can diminish the levels of mature miRNA, let-7b and mir-30a. Overall, our study uncovers, for the first time, Dicer1 and transporter proteins as clients of Aha1 and HSP90.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:26.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yen-Yu Yang
SpeciesList	scientific name: Pan troglodytes (Chimpanzee); NCBI TaxID: 9598; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-07 00:35:00	ID requested
1	2022-11-19 14:27:44	announced
⏵ 2	2023-11-14 08:53:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: HSP90, Dicer1, APEX, Proximity labeling, Aha1

Yinsheng Wang
contact affiliation	Department of Chemistry, University of California Riverside
contact email	yinsheng@ucr.edu
lab head
Yen-Yu Yang
contact affiliation	UC Riverside
contact email	yyang227@ucr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD028980

PRIDE project URI

• PRIDE
  1. PXD028980
     1. Label: PRIDE project
     2. Name: Proximity Proteomics Revealed Dicer1 and Transporters as Client Proteins of Aha1