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PXD028975

PXD028975 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEngineering Extracellular Vesicles by Three-dimensional Dynamic Culture of Human Mesenchymal Stem Cells
DescriptionExtracellular vesicles (EVs) contain proteins, enzymes and metabolites that contribute to the therapeutic potential of human mesenchymal stem cells (hMSCs). However, scale-up production of hMSC EVs has become a major challenge. In current study, hMSCs were grown as 3D aggregates under wave motion to promote EV secretion (3D EVs). mRNA sequencing reveals global transcriptome alterations (e.g., upregulated Wnt, TNF, and Hippo signaling and downregulated cellular senescence) for 3D aggregates. Compared to 2D EVs, the quantity of 3D EVs was enhanced significantly with smaller size, higher miR-21 and miR-22 expression, and the altered protein cargo revealed by proteomics. 3D hMSC aggregates promote activation of the endosomal sorting complexes required for transport (ESCRT) pathway and ESCRT-independent pathway. In addition, 3D EVs rejuvenated stem cells expressing cellular senescence and modulated immune response determined by T lymphocyte and macrophage phenotype assays. In summary, this study provides a promising strategy for high-quality EV production from hMSCs with enhanced therapeutic potentials.
HostingRepositoryPRIDE
AnnounceDate2022-08-12
AnnouncementXMLSubmission_2022-08-12_00:04:04.154.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD028975
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterli sun
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-07 00:24:05ID requested
12022-08-12 00:04:04announced
Publication List
Yuan X, Sun L, Jeske R, Nkosi D, York SB, Liu Y, Grant SC, Meckes DG, Li Y, Engineering extracellular vesicles by three-dimensional dynamic culture of human mesenchymal stem cells. J Extracell Vesicles, 11(6):e12235(2022) [pubmed]
Keyword List
submitter keyword: human mesenchymal stem cells, extracellular vesicles, 3D aggregates, biogenesis, rejuvenation, immunomodulation
Contact List
David Meckes
contact affiliationFlorida State University college of medicine
contact emailDavid.Meckes@med.fsu.edu
lab head
li sun
contact affiliationFlorida State University
contact emailli.sun@med.fsu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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