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PXD028948

PXD028948 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleC. difficle p-cresol production
DescriptionTo identify the cellular localisation of the HpdBCA decarboxylase complex, a plasmid based HpdB-SNAP-tag translational fusion was constructed using a C. difficile compatible plasmid (pMTL84151) under control of the hpdBCA promoter. The hpdB coding sequence omitting the stop codon, was fused via a linker to a SNAP-tag (Table 1). Confirmation of HpdB linked to the SNAP-tag was carried out via western blot, and mass spectrometry, in which we identified six peptides, four with 100% identity and two with 99% identity unique to HpdB. Localisation of HpdB was visualised by confocal microscopy in 630Δerm and the p-cresol deficient mutant (hpdC::CT) (carrying the HpdB-SNAP-tag fusion (PhpdB-CDS-SNAP), in the presence and absence of p-HPA) to induce HpdBCA production.
HostingRepositoryPRIDE
AnnounceDate2021-11-02
AnnouncementXMLSubmission_2021-11-02_04:16:20.249.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSteven Lynham
SpeciesList scientific name: Clostridioides difficile ATCC 43255; NCBI TaxID: 499175;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-05 02:47:48ID requested
12021-11-02 04:16:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: HpdBCA decarboxylase
Contact List
Dr Lisa Dawson
contact affiliationInfection Biology – LSHTM, Keppel street, WC1E 7HT
contact emaillisa.dawson@lshtm.ac.uk
lab head
Steven Lynham
contact affiliationKing's College London
contact emailsteven.lynham@kcl.ac.uk
dataset submitter
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