PXD028934

PXD028934 is an original dataset announced via ProteomeXchange.

Title	Analysis of newly synthesized proteins more sensitive to Proteinase K using pulse SILAC and control sample for pulse SILAC experiment in which yeast cells were heat shock for 15 min and supernatant fractions analyzed (Experiment 1)
Description	Accurate and efficient folding of nascent protein sequences into their native state requires support from the protein homeostasis network. Herein we probed which newly translated proteins are thermo-sensitive to infer which polypeptides require more time to fold within the proteome. Specifically, we determined which of these proteins were more susceptible to misfolding and aggregation under heat stress using pulse SILAC coupled mass spectrometry. These proteins are abundant, short, and highly structured. Notably these proteins display a tendency to form β-sheet secondary structures, a configuration which typically requires more time for folding, and were enriched for Hsp70/Ssb and TRiC/CCT binding motifs, suggesting a higher demand for chaperone-assisted folding. These polypeptides were also more often components of stable protein complexes in comparison to other proteins. Combining this evidence suggests that a specific subset of newly translated proteins in the cell requires more time following synthesis to reach a state less prone to aggregation upon stress.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:08.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibault Mayor
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-04 10:02:51	ID requested
1	2022-08-10 16:47:56	announced
⏵ 2	2023-11-14 08:53:09	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: protein homeostasis, yeast,pulse SILAC, Limited Proteolysis

Thibault Mayor
contact affiliation	The University of British Columbia
contact email	mayor@mail.ubc.ca
lab head
Thibault Mayor
contact affiliation	University of British Columbia
contact email	mayor@mail.ubc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028934
     1. Label: PRIDE project
     2. Name: Analysis of newly synthesized proteins more sensitive to Proteinase K using pulse SILAC and control sample for pulse SILAC experiment in which yeast cells were heat shock for 15 min and supernatant fractions analyzed (Experiment 1)