PXD028919

PXD028919 is an original dataset announced via ProteomeXchange.

Title	Basolateral Protein Scribble Provides Phosphatase PP1 to a Signaling Network Maintaining Apicobasal Polarity
Description	Scribble, a member of the LAP protein family, contributes to the apicobasal polarity (ABP) of epithelial cells. The LAP unique region (LUR) of these proteins is essential for ABP function, and includes a highly conserved Leucine Reach Repeat (LRR) domain. Here we study how the LRR domain of Scribble maintains ABP. We show that its concave surface participates in three types of mutually exclusive interactions 1. homodimerization serving as an auto-inhibitory mechanism, 2. interactions with a diverse set of polarity proteins, such as Llgl1, Llgl2, EPB41L2 and EPB41L5, which produce distinct multiprotein complexes, and 3. a direct interaction with the protein phosphatase, PP1, which forms a dimeric LRR domain-PP1 complex. Our results suggest that the latter complex maintains PP1 in the basolateral cell cortex in close proximity to PP1 targets. Such organization generates a dynamic network where PP1 could be immediately dispatched from the Scribble-PP1 complex to particular protein ligands. This mechanism for controlling dephosphorylation kinetics of phosphorylated proteins could be universal for all members of the LAP protein family, which includes Erbin, Lano, and the neuron-specific protein, Densin-180.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_05:48:22.082.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028919
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Indrajyoti Indra
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-04 10:00:50	ID requested
⏵ 1	2021-11-03 05:48:23	announced

Troyanovsky RB, Indra I, Kato R, Mitchell BJ, Troyanovsky SM, Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity. J Biol Chem, 297(5):101289(2021) [pubmed]

submitter keyword: Scribble, Apicobasal Polarity, Phosphatase PP1

Sergey Troyanovsky
contact affiliation	Department of Dermatology, Northwestern University, USA
contact email	s-troyanovsky@northwestern.edu
lab head
Indrajyoti Indra
contact affiliation	Northwestern University
contact email	indrajyoti.indra@northwestern.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028919
     1. Label: PRIDE project
     2. Name: Basolateral Protein Scribble Provides Phosphatase PP1 to a Signaling Network Maintaining Apicobasal Polarity