PXD028902

PXD028902 is an original dataset announced via ProteomeXchange.

Title	Temporal and site-specific ADP-ribosylation dynamics upon different genotoxic stresses
Description	The DNA damage response revolves around transmission of information via post-translational modifications, including reversible protein ADP-ribosylation. Here, we applied a mass spectrometry-based Af1521 enrichment technology for the identification and quantification of ADP-ribosylation sites as a function of various DNA damage stimuli and time. In total, we detected 1,681 ADP-ribosylation sites residing on 716 proteins in U2OS cells, and determined their temporal dynamics after exposure to the genotoxins H2O2 and MMS. Intriguingly, we observed a widespread but low-abundant serine ADP-ribosylation response at the earliest time point, with later time points centered on increased modification of the same sites. This suggests that early serine ADP-ribosylation events may serve as a platform for an integrated signal response. While treatment with H2O2 and MMS induced homogenous ADP-ribosylation responses, we observed temporal differences in the ADP-ribosylation site abundancies. Exposure to MMS-induced alkylating stress induced the strongest ADP-ribosylome response after 30 minutes prominently modifying proteins involved in RNA processing, whereas in response to H2O2-induced oxidative stress ADP-ribosylation peaked after 60 minutes, while mainly modifying proteins involved in DNA damage pathways. Collectively, the dynamic ADP-ribosylome presented here provides valuable insight into the temporal cellular regulation of ADP-ribosylation in response to DNA damage.
HostingRepository	PRIDE
AnnounceDate	2023-05-14
AnnouncementXML	Submission_2023-05-14_14:19:03.280.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SaraLarsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-04 04:44:57	ID requested
1	2021-11-04 00:31:08	announced
⏵ 2	2023-05-14 14:19:04	announced	2023-05-14: Updated project metadata.

Buch-Larsen SC, Rebak AKLFS, Hendriks IA, Nielsen ML, Temporal and Site-Specific ADP-Ribosylation Dynamics upon Different Genotoxic Stresses. Cells, 10(11):(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: proteomics, mass spectrometry, PARP, Post-translational modification, DNA damage, Af1521,ADP-ribosylation

Michael LundNielsen
contact affiliation	Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
contact email	michael.lund.nielsen@cpr.ku.dk
lab head
SaraLarsen
contact affiliation	NNF Center for Protein Research
contact email	sara.larsen@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD028902

PRIDE project URI

• PRIDE
  1. PXD028902
     1. Label: PRIDE project
     2. Name: Temporal and site-specific ADP-ribosylation dynamics upon different genotoxic stresses