PXD028897

PXD028897 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the membrane-enriched fraction of trypomastigostes released from wild type, LAMP1/2 KO or LAMP2 KO fibroblasts
Description	Trypanosoma cruzi invades non-professional phagocytic cells by subverting their membrane repair process, which is dependent on membrane injury and cell signaling, intracellular calcium increase and lysosome recruitment. Cells lacking Lysosome Associated Membrane Proteins 1 and 2 are less permissive to parasite invasion, however more prone to parasite intracellular multiplication. Several passages through a different intracellular environment can significantly change T. cruzi’s gene expression profile. We evaluated whether one single passage through LAMP deficient (KO) or wild type (WT) fibroblasts could influence invasion ability of T. cruzi Y strain trypomastigotes in L6 myoblasts and WT fibroblasts. Our results regarding their biological behavior clearly indicated that parasites released from WT cells (TcY-WT), LAMP1/2 KO cells (TcY-L1/2-/-) or LAMP2 KO (TcY-L2-/-) cells are distinct from each other. Since these alterations most likely would reflect differences among parasite surface molecules, we also evaluated their proteome. We identified few protein complexes, membrane and secreted proteins regulated in our dataset. Among those, some members of MASP, mucins, trans-sialidases and gp63 proteins family, which are known to play an important role during parasite infection and could correlate to TcY-WT, TcY-L1/2-/- and TcY-L2-/- biological behavior.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_16:27:53.595.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anny Carolline Silva Oliveira
SpeciesList	scientific name: Trypanosoma cruzi; NCBI TaxID: 5693;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-04 04:43:51	ID requested
⏵ 1	2022-02-16 16:27:54	announced

Oliveira ACS, Rezende L, Gorshkov V, Melo-Braga MN, Verano-Braga T, Fernandes-Braga W, Guadalupe JLM, de Menezes GB, Kjeldsen F, de Andrade HM, Andrade LO, Residence in a LAMP-Deficient Intracellular Environment. Front Cell Infect Microbiol, 11():788482(2021) [pubmed]

submitter keyword: Trypanosoma cruzi, TMT labeling, membrane-enriched fraction

Luciana de Oliveira Andrade
contact affiliation	Department of Morphology, Cell and Molecular Biology Laboratory (LBCM),Biological Sciences Institute (ICB), Federal University of Minas Gerais (UFMG), Brazil
contact email	lucianaandrade@ufmg.br
lab head
Anny Carolline Silva Oliveira
contact affiliation	Federal University of Minas Gerais
contact email	1990.annyoliveira@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028897

PRIDE project URI

• PRIDE
  1. PXD028897
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the membrane-enriched fraction of trypomastigostes released from wild type, LAMP1/2 KO or LAMP2 KO fibroblasts