PXD028878

PXD028878 is an original dataset announced via ProteomeXchange.

Title	An Evolutionarily Conserved Regulatory Pathway of Muscle Mitochondrial Network Organization
Description	Mitochondrial networks provide coordinated energy distribution throughout muscle cells. However, pathways specifying mitochondrial network-type separately from contractile fiber-type remain unclear. Here, we show that natural energetic demands placed on Drosophila melanogaster muscles yield native cell-types among which contractile and mitochondrial network-types are regulated independently. Proteomic analyses of indirect flight, jump, and leg muscles together with muscles misexpressing known fiber-type specification factor salm identified transcription factors H15 and cut as potential mitochondrial network regulators. We demonstrate H15 operates downstream of salm regulating flight muscle contractile and mitochondrial network-type. Conversely, H15 regulates mitochondrial network configuration but not contractile type in jump and leg muscles. Further, we find that cut regulates salm expression in flight muscles and mitochondrial network configuration in leg muscles. These data indicate cell type-specific regulation of muscle mitochondrial network organization separately from contractile type, mitochondrial content, and mitochondrial size through an evolutionarily conserved pathway involving cut, salm, and H15.
HostingRepository	MassIVE
AnnounceDate	2022-10-13
AnnouncementXML	Submission_2022-10-13_07:28:32.570.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Angel Aponte
SpeciesList	scientific name: Drosophila melanogaster; common name: fruit fly; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-30 08:56:45	ID requested
⏵ 1	2022-10-13 07:28:32	announced

no publication

submitter keyword: Mitochondria, TMT

Brian Glancy
contact affiliation	NIH/NHLBI
contact email	Brian.Glancy@nih.gov
lab head
Angel Aponte
contact affiliation	NIH/NHLBI
contact email	apontea@nhlbi.nih.gov
dataset submitter

MassIVE dataset URI

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