PXD028873

PXD028873 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics reveals regulation of secondary metabolites in Mahonia bealei under ultraviolet-B radiation
Description	Mahonia Bealei was used as a traditional Chinese medicine for its high alkaloid content. Previous research found that alkaloid and flavonoid contents in the M. bealei leaves increased under combinatory treatments of ultraviolet B and dark. In order to explore the underlying response mechanism, TiO2 material enrichment and mass-based label-free quantitative proteomics techniques were used for phosphoproteomics analysis of M. bealei leaves under ultraviolet B. ATP content, photosynthetic pigment content, and some enzymatic/non-enzymatic indicators increased in the leaves of M. bealei under UV-B radiation. Phosphoproteomics study found that under the UV-B radiation, phosphoproteins related to MAPK signal transduction and plant hormone brassinosteroid signaling pathway were varied greatly. Phosphoproteins related to photosynthesis, glycolysis, tricarboxylic acid cycle, and amino acid synthesis/metabolism pathway were also significantly changed. These results suggested that the ultraviolet B radiation activated oxidative stress system, MAPK signal transduction pathway, and photosynthetic energy metabolism pathway. These changes are important for the redox reactions in secondary metabolism and the accumulation of secondary metabolites in M. bealei leaves under UV-B radiation.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_16:01:32.072.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amin Liu
SpeciesList	scientific name: Mahonia; NCBI TaxID: 2750454;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-30 03:35:54	ID requested
⏵ 1	2022-02-16 16:01:32	announced

Liu A, Liu S, Li Y, Tao M, Han H, Zhong Z, Zhu W, Tian J, Exposed to Ultraviolet-B Radiation. Front Plant Sci, 12():794906(2021) [pubmed]

submitter keyword: Phosphoproteomics

Mahonia Bealei

Ultraviolet-B Radiation

Abiotic stress

secondary metabolism

Amin Liu
contact affiliation	College of Biomedical Engineering & Instrument Science, Zhejiang University
contact email	755819869@qq.com
lab head
Amin Liu
contact affiliation	Zhejiang university
contact email	755819869@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028873
     1. Label: PRIDE project
     2. Name: Phosphoproteomics reveals regulation of secondary metabolites in Mahonia bealei under ultraviolet-B radiation