PXD028858

PXD028858 is an original dataset announced via ProteomeXchange.

Title	Histone H3 lysine 4 trimethylation regulates RNA polymerase II promoter-proximal pause-release
Description	Trimethylation of histone H3 lysine 4 (H3K4me3) is associated with transcriptional start sites and proposed to regulate transcription initiation. However, redundant functions of the H3K4 SET1/COMPASS methyltransferase complexes complicate elucidation of the specific role of H3K4me3 in transcriptional regulation. Here, we show that acute ablation of shared subunits of the SET1/COMPASS complexes leads to complete loss of all H3K4 methylation. H3K4me3 turnover occurs more rapidly than H3K4me1 and H3K4me2 and is dependent on KDM5 demethylases. Surprisingly, acute loss of H3K4me3 does not have detectable effects on transcriptional initiation but leads to a widespread decrease in transcriptional output, an increase in RNA polymerase II (RNAPII) pausing and slower elongation. Our study demonstrates a distinct role for H3K4me3 in transcriptional pause-release and elongation rather than transcriptional initiation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:16.406.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Shliaha
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-29 14:18:17	ID requested
1	2023-03-14 07:22:34	announced
⏵ 2	2023-11-14 08:44:17	announced	2023-11-14: Updated project metadata.

Wang H, Fan Z, Shliaha PV, Miele M, Hendrickson RC, Jiang X, Helin K, H3K4me3 regulates RNA polymerase II promoter-proximal pause-release. Nature, 615(7951):339-348(2023) [pubmed]

submitter keyword: histone,RNA polymerase II

Ronald Hendrickson
contact affiliation	Microchemistry and Proteomics Core Facility, Memorial Sloan KetteringCancer Center, New York, NY 10065, USA (lab head)
contact email	hendricr@mskcc.org
lab head
Pavel Shliaha
contact affiliation	Syddansk University
contact email	pavels@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD028858

PRIDE project URI

• PRIDE
  1. PXD028858
     1. Label: PRIDE project
     2. Name: Histone H3 lysine 4 trimethylation regulates RNA polymerase II promoter-proximal pause-release