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PXD028840

PXD028840 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA method for independent estimation of false localisation rate for phosphoproteomics
DescriptionPhosphoproteomics methods are commonly employed in labs to identify and quantify the sites of phosphorylation on proteins. In recent years, various software tools have been developed, incorporating scores or statistics related to whether a given phosphosite has been correctly identified, or to estimate the global false localisation rate (FLR) within a given data set for all sites reported. These scores have generally been calibrated using synthetic data sets, and their statistical reliability on real datasets is largely unknown. As a result, there is considerable problem in the field of reporting incorrectly localised phosphosites, due to inadequate statistical control. In this work, we develop the concept of using scoring and ranking modifications on a decoy amino acid, i.e. one that cannot be modified, to allow for independent estimation of global FLR. We test a variety of different amino acids to act as the decoy, on both synthetic and real data sets, demonstrating that the amino acid selection can make a substantial difference to the estimated global FLR. We conclude that while several different amino acids might be appropriate, the most reliable FLR results were achieved using alanine and leucine as decoys, although we have a preference for alanine due to the risk of potential confusion between leucine and isoleucine amino acids. We propose that the phosphoproteomics field should adopt the use of a decoy amino acid, so that there is better control of false reporting in the literature, and in public databases that re-distribute the data.
HostingRepositoryPRIDE
AnnounceDate2022-06-09
AnnouncementXMLSubmission_2022-06-09_04:29:28.966.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD028840
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKerry Ramsbottom
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion ETD; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-29 06:13:18ID requested
12022-06-09 04:29:29announced
Publication List
Ramsbottom KA, Prakash A, Riverol YP, Camacho OM, Martin MJ, Vizca, í, no JA, Deutsch EW, Jones AR, Method for Independent Estimation of the False Localization Rate for Phosphoproteomics. J Proteome Res, 21(7):1603-1615(2022) [pubmed]
Keyword List
submitter keyword: Phosphorylation, false localisation
Contact List
Prof. Andrew R Jones
contact affiliationBiochemistry & Systems Biology, Universiy of Liverpool, UK
contact emailAndrew.Jones@liverpool.ac.uk
lab head
Kerry Ramsbottom
contact affiliationUniversity of Liverpool
contact emailkramsb@liverpool.ac.uk
dataset submitter
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Dataset FTP location
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Repository Record List
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