PXD028835

PXD028835 is an original dataset announced via ProteomeXchange.

Title	I.e. mouse ovary palmitoylation protein LC-MSMS
Description	To identify a role for protein palmitoylation in ovarian of DHEA-induced PCOS mice model. First, we used DHEA to induce a PCOS mice model，hematoxylin-eosin (H&E) staining sections form the ovaries of the Control and DHEA groups.The mice oral gavage with DHEA disrupted ovarian morphology,the estrous cycle, and hormone profilein DHEA-induced mouse. The three pairs of ovary for each tube are mixed together as a group were lysed and divided into 2 fractions. The -HAM condition served as a negative control and the +HAM sample positive control.In the +HAM sample, the palmitate residue was cleaved off and exchanged with biotin. After the ABE reaction was completed, streptavidin beads were used to enrich for biotinylated proteins. Proteins enriched from +HAM and −HAM conditions were identified using mass spectrometry (MS).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:56.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tang Ting
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	palmitoylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-29 01:41:06	ID requested
1	2023-03-10 19:16:47	announced
⏵ 2	2023-11-14 08:28:57	announced	2023-11-14: Updated project metadata.

Xue T, Zhao S, Zhang H, Tang T, Zheng L, Jing J, Ge X, Ma R, Ma J, Ren X, Jueraitetibaike K, Guo Z, Chen L, Yao B, depalmitoylation participates in the pathogenesis of hyperandrogenism. iScience, 26(3):106131(2023) [pubmed]

submitter keyword: I.e. mouse ovary， palmitoylation protein， LC-MSMS

Bing Yao
contact affiliation	Department of Reproductive Medicine, Affiliated Jinling Hospital , Nanjing Medical University
contact email	yaobing@nju.edu.cn
lab head
Tang Ting
contact affiliation	Center of Reproductive Medicine, Nanjing Jinling Hospital
contact email	tangting2019@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD028835

PRIDE project URI

• PRIDE
  1. PXD028835
     1. Label: PRIDE project
     2. Name: I.e. mouse ovary palmitoylation protein LC-MSMS