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PXD028834

PXD028834 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal profiling of the proteomic changes associated with the post-testicular maturation of mouse spermatozoa
DescriptionSpermatozoa acquire fertilization potential during passage through a highly specialized region of the extra-testicular ductal system known as the epididymis. In the absence of de novo gene transcription or protein translation, this functional transformation is extrinsically driven via the exchange of varied macromolecular cargo between spermatozoa and the surrounding luminal plasma. Key among these changes is a substantive remodeling of the sperm proteomic architecture, the scale of which has yet to be fully resolved. Here, we have exploited quantitative mass spectrometry-based proteomics to define the extent of changes associated with the maturation of mouse spermatozoa; reporting the identity of an unprecedented >6,000 proteins, encompassing the selective loss and gain of several hundred proteins. Further, we demonstrate epididymal driven activation of RHOA mediated signaling pathways is an important component of sperm maturation. These data contribute molecular insights into the complexity of proteomic changes associated with epididymal sperm maturation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:11:33.855.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD028834
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Skerrett-Byrne
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-29 01:41:05ID requested
12022-11-20 22:01:55announced
22023-11-14 08:11:36announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD028834;
Skerrett-Byrne DA, Anderson AL, Bromfield EG, Bernstein IR, Mulhall JE, Schjenken JE, Dun MD, Humphrey SJ, Nixon B, Global profiling of the proteomic changes associated with the post-testicular maturation of mouse spermatozoa. Cell Rep, 41(7):111655(2022) [pubmed]
Keyword List
submitter keyword: RHOA, proteomics, mass spectrometry,Acrosome reaction, sperm maturation, epididymis, male fertility, sperm proteome, spermatozoa
Contact List
Brett Nixon
contact affiliationReproductive Science Group, Priority Research Centre for Reproductive Science School of Environmental and Life Sciences College of Engineering, Science and Environment
contact emailBrett.Nixon@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliationThe University of Newcastle
contact emailDavid.Skerrett-Byrne@newcastle.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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