PXD028834 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global profiling of the proteomic changes associated with the post-testicular maturation of mouse spermatozoa |
Description | Spermatozoa acquire fertilization potential during passage through a highly specialized region of the extra-testicular ductal system known as the epididymis. In the absence of de novo gene transcription or protein translation, this functional transformation is extrinsically driven via the exchange of varied macromolecular cargo between spermatozoa and the surrounding luminal plasma. Key among these changes is a substantive remodeling of the sperm proteomic architecture, the scale of which has yet to be fully resolved. Here, we have exploited quantitative mass spectrometry-based proteomics to define the extent of changes associated with the maturation of mouse spermatozoa; reporting the identity of an unprecedented >6,000 proteins, encompassing the selective loss and gain of several hundred proteins. Further, we demonstrate epididymal driven activation of RHOA mediated signaling pathways is an important component of sperm maturation. These data contribute molecular insights into the complexity of proteomic changes associated with epididymal sperm maturation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:11:33.855.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD028834 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | David Skerrett-Byrne |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-29 01:41:05 | ID requested | |
1 | 2022-11-20 22:01:55 | announced | |
⏵ 2 | 2023-11-14 08:11:36 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD028834; |
Skerrett-Byrne DA, Anderson AL, Bromfield EG, Bernstein IR, Mulhall JE, Schjenken JE, Dun MD, Humphrey SJ, Nixon B, Global profiling of the proteomic changes associated with the post-testicular maturation of mouse spermatozoa. Cell Rep, 41(7):111655(2022) [pubmed] |
Keyword List
submitter keyword: RHOA, proteomics, mass spectrometry,Acrosome reaction, sperm maturation, epididymis, male fertility, sperm proteome, spermatozoa |
Contact List
Brett Nixon |
contact affiliation | Reproductive Science Group, Priority Research Centre for Reproductive Science School of Environmental and Life Sciences College of Engineering, Science and Environment |
contact email | Brett.Nixon@newcastle.edu.au |
lab head | |
David Skerrett-Byrne |
contact affiliation | The University of Newcastle |
contact email | David.Skerrett-Byrne@newcastle.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028834
- Label: PRIDE project
- Name: Global profiling of the proteomic changes associated with the post-testicular maturation of mouse spermatozoa