PXD028830

PXD028830 is an original dataset announced via ProteomeXchange.

Title	In Situ Proximity Labeling Identifies Lewy Pathology Molecular Interactions in the Human Brain
Description	The intracellular misfolding and accumulation of alpha-synuclein into structures collectively called LP is a central phenomenon for the pathogenesis of synucleinopathies including Parkinson’s disease (PD) and Dementia with Lewy Bodies (DLB). Understanding the molecular architecture of LP is crucial for understanding synucleinopathy disease origins and progression. Here we used a technique called biotinylation by antibody recognition (BAR) to label total (BAR-SYN1) and pathological alpha-synuclein (BAR-PSER129) in situ for subsequent mass spectrometry analysis. This technique has broad potential to help understand the phenomenon of LP in primary human tissue and animal models.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:34:40.570.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028830
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bryan Killinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-29 01:33:19	ID requested
⏵ 1	2022-02-22 04:34:41	announced

Killinger BA, Marshall LL, Chatterjee D, Chu Y, Bras J, Guerreiro R, Kordower JH, In situ proximity labeling identifies Lewy pathology molecular interactions in the human brain. Proc Natl Acad Sci U S A, 119(5):(2022) [pubmed]

submitter keyword: Alpha-synuclein, neuropathology, lewy pathology, in situ, biotinylation by antigen recognition

Bryan Andrew Killinger
contact affiliation	Graduate College and Department of Neurology Rush University Medical Center
contact email	bryan_killinger@rush.edu
lab head
Bryan Killinger
contact affiliation	Rush University Medical Center
contact email	bryan_killinger@rush.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028830

PRIDE project URI

• PRIDE
  1. PXD028830
     1. Label: PRIDE project
     2. Name: In Situ Proximity Labeling Identifies Lewy Pathology Molecular Interactions in the Human Brain