PXD028828

PXD028828 is an original dataset announced via ProteomeXchange.

Title	APC/CCdc20 dependent degradation of Clb4 is all there is to astral microtubule stabilization
Description	Coordination between sister chromatid separation and segregation is crucial for accurate chromosome partitioning to the offspring. Key to this process is the ability of mitotic spindle microtubules to respond to different molecular signals and remodel their dynamics accordingly. Based on their function, spindle microtubules are conventionally divided into three classes: kinetochore, interpolar and astral microtubules (kMTs, iMTs and aMTs, respectively). While different mechanisms have been proposed to control kMT and iMT dynamics, aMT regulation remains elusive. We here show that aMT dynamics are tightly regulated. aMTs remain unstable up to metaphase to control spindle orientation and are stabilized at anaphase onset. Necessary and sufficient for this stabilization is the degradation of the mitotic cyclin Clb4 mediated by the Anaphase Promoting Complex in combination with its activator subunit Cdc20 (APC/CCdc20). Our results contribute to delineate a comprehensive picture of late mitotic events, where individual steps of the signalling cascade initiated by APC/CCdc20 activation and culminating in cohesin cleavage time the final stages of mitosis by sequentially modulating the dynamics of the three classes of spindle microtubules.
HostingRepository	PRIDE
AnnounceDate	2022-10-19
AnnouncementXML	Submission_2022-10-18_19:18:12.474.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JiamingLi
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-29 01:33:06	ID requested
⏵ 1	2022-10-18 19:18:12	announced

Dataset with its publication pending

submitter keyword: astral microtubule stabilization

Steven P.Gygi
contact affiliation	Department of Cell Biology, Harvard Medical School
contact email	steven_gygi@hms.harvard.edu
lab head
JiamingLi
contact affiliation	Harvard Medical School
contact email	jiaming_li@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028828
     1. Label: PRIDE project
     2. Name: APC/CCdc20 dependent degradation of Clb4 is all there is to astral microtubule stabilization