PXD028826

PXD028826 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	PRM analysis of phosphopeptides of Nab2, Foxk1, HURP, ERK1/2, NPM, and Hspa4 from ΔB-Raf:ER cells.
Description	ΔB-Raf:ER cells were treated with U0126 or 4-HT for 30 min in four biological replicates. Total proteins from cell lysates were digested with Typsin/Lys-C mix, and phosphopeptides were enriched by IMAC using Fe-NTA columns. After data-dependent LC-MS/MS analysis, phosphopeptides of Nab2, Foxk1, HURP, ERK1/2, NPM, and Hspa4 were selected and quantified by targeted MS using the PRM method. Targeted MS/MS scans were acquired by a time-scheduled inclusion list at a resolution of 70,000, an AGC target of 2e5, an isolation window of 2.0 m/z, a maximum injection time of 500 ms, and a normalized collision energy of 27. Time alignment and relative quantification of the transitions were performed using Skyline software.
HostingRepository	jPOST
AnnounceDate	2022-03-03
AnnouncementXML	Submission_2022-09-18_02:22:41.979.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-29 00:50:08	ID requested
1	2022-03-03 06:46:47	announced
⏵ 2	2022-09-18 02:22:42	announced	2022-09-18: Updated FTP location.

Publication List

Yoshikawa H, Nishino K, Kosako H, Identification and validation of new ERK substrates by phosphoproteomic technologies including Phos-tag SDS-PAGE. J Proteomics, 258():104543(2022) [pubmed]

Yoshikawa H, Nishino K, Kosako H, Identification and validation of new ERK substrates by phosphoproteomic technologies including Phos-tag SDS-PAGE. J Proteomics, 258():104543(2022) [pubmed]

Keyword List

submitter keyword: PRM, phosphopeptides, ERK, ΔB-Raf:ER cells

submitter keyword: PRM, phosphopeptides, ERK, ΔB-Raf:ER cells

Contact List

Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001341/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001341/