PXD028798

PXD028798 is an original dataset announced via ProteomeXchange.

Title	Analysis of proteins that bind promoter of the PDR5 multidrug transporter gene in Saccharomyces cerevisiae using minichromosome isolation and LC-MS/MS
Description	The Pleiotropic Drug Resistance (PDR) network is central to the drug response in fungi, and its overactivation is associated with drug resistance. However, gene regulation of the PDR network is not well understood. Here, we established a method to identify proteins that bind promoter of the PDR5 multidrug transporter gene in Saccharomyces cerevisiae using minichromosome isolation and SILAC-based quantitative proteomics, and identified the SWI/SNF chromatin remodelling complex as a PDR5 promoter-binding complex. We also purified the SWI/SNF complex from S. cerevisiae by immunoprecipitating Flag-tagged Snf6, a subunit of SWI/SNF, and identified the subunits of SWI/SNF and its binding proteins by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_02:35:35.047.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Stead
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-28 03:07:42	ID requested
⏵ 1	2022-02-22 02:35:35	announced

Dataset with its publication pending

submitter keyword: Saccharomyces cerevisiae, minichromosome, promoter-binding proteins, immunoprecipitation, LC-MSMS

Takashi Kubota
contact affiliation	Institute of Medical Sciences University of Aberdeen Foresterhill Aberdeen AB25 2ZD Scotland UK
contact email	t.kubota@abdn.ac.uk
lab head
David Stead
contact affiliation	University of Aberdeen
contact email	d.stead@abdn.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028798
     1. Label: PRIDE project
     2. Name: Analysis of proteins that bind promoter of the PDR5 multidrug transporter gene in Saccharomyces cerevisiae using minichromosome isolation and LC-MS/MS