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PXD028789

PXD028789 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomic analysis of the adaption of epididymal epithelial cells to corticosterone challenge
DescriptionBackground: The epididymis has long been of interest owing to its role in promoting the functional maturation of the male germline. More recent evidence has also implicated the epididymis as an important sensory tissue responsible for remodeling of the sperm epigenome, both under physiological conditions and in response to diverse forms of environmental stress. Despite this knowledge, the intricacies of the molecular pathways involved in regulating the adaptation of epididymal tissue to paternal stressors remains to be fully resolved. Objective: The overall objective of this study was to investigate the direct impact of corticosterone challenge on a tractable epididymal epithelial cell line (i.e., mECap18 cells) in terms of driving adaptation of the cellular proteome and phosphoproteome signaling networks. Materials and methods: The newly developed phosphoproteomic platform EasyPhos coupled with sequencing via an Orbitrap Exploris 480 mass spectrometer was applied to survey global changes in the mECap18 cell (phospho)proteome resulting from sub-chronic (10-day) corticosterone challenge. Results: The imposed corticosterone exposure regimen elicited relatively subtle modifications of the global mECap18 proteome [i.e., only 73/4171 (~1.8%) proteins displayed altered abundance]. By contrast, ~15% of the mECap18 phosphoproteome was substantially altered following corticosterone challenge. In-silico analysis of the corresponding parent proteins revealed an activation of pathways linked to DNA damage repair and oxidative stress responses as well as a reciprocal inhibition of pathways associated with organismal death. Corticosterone challenge also induced the phosphorylation of several proteins linked to the biogenesis of microRNAs. Accordingly, orthogonal validation strategies confirmed an increased in DNA damage, which was ameliorated upon selective kinase inhibition, and an altered abundance profile of a subset of microRNAs in corticosterone-treated cells. Conclusions: Together, these data confirm that epididymal epithelial cells are reactive to corticosterone challenge and that their response is tightly coupled to the opposing action of cellular kinases and phosphatases.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:35:19.332.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD028789
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Skerrett-Byrne
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-27 06:50:02ID requested
12024-04-08 01:35:36announced
22024-10-22 06:35:20announced2024-10-22: Updated project metadata.
Publication List
Skerrett-Byrne DA, Stanger SJ, Trigg NA, Anderson AL, Sipil, ä P, Bernstein IR, Lord T, Schjenken JE, Murray HC, Verrills NM, Dun MD, Pang TY, Nixon B, Phosphoproteomic analysis of the adaption of epididymal epithelial cells to corticosterone challenge. Andrology, 12(5):1038-1057(2024) [pubmed]
10.1111/andr.13636;
Keyword List
submitter keyword: proteomics,Cellular signaling, stress response, corticosterone, sperm maturation, phosphoproteomics, epididymis
Contact List
Brett Nixon
contact affiliationPriority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, 2308, Australia.
contact emailbrett.nixon@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliationHelmholtz Zentrum München
contact emailDavid.Skerrett-Byrne@newcastle.edu.au
dataset submitter
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Dataset FTP location
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