PXD028785

PXD028785 is an original dataset announced via ProteomeXchange.

Title	Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection
Description	Subversion of the host cytoskeleton is a critical virulence mechanism used by a variety of intracellular bacterial pathogens during their infectious life cycles. Growing evidence suggests that the tactics employed by pathogens are surprisingly diverse. Using proteomic and biochemical methods, we demonstrate that the S. Tm type III effector protein SopB potently interacts specifically with host cell IFs vimentin. After host cell entry, Salmonella replicates in Salmonella containing vacuoles (SCVs), which accumulate a dense meshwork of vimentin through the Salmonella SPI-1 type III secretion effector SopB. We also demonstrate an important role for SopB-vimentin interaction, in the cellular responses including pro-inflammatory cytokine production induced by Salmonella. Vimentin interacts with the N-terminus of the S. Tm T3SS effector protein SopB. Vimentin and its interaction with SopB are dispensable for S. Tm invasion, but are required for stable formation of SCVs which are essential for bacteria replication. Moreover, SopB lacking of N-terminus cdc42-binding domain loses the interaction with vimentin, suggesting that the small GTPase is critical in SopB triggered vimentin recruitment to form integrative SCVs. These findings establish that SCVs integrity of the bacterium specifically requires intermediate filaments vimentin, which is a prerequisite for highly efficient S. Tm replication.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:30.377.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuangshuang Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-27 06:42:05	ID requested
1	2023-03-11 10:19:11	announced
⏵ 2	2023-11-14 08:43:36	announced	2023-11-14: Updated project metadata.

Zhao S, Xu Q, Cui Y, Yao S, Jin S, Zhang Q, Wen Z, Ruan H, Liang X, Chao Y, Gong S, Sansonetti P, Wei K, Tang H, Jiu Y, Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection. Nat Commun, 14(1):478(2023) [pubmed]

submitter keyword: Human, LC-MS, vimentin, S.Tm

Yaming Jiu
contact affiliation	Institut Pasteur of Shanghai
contact email	ymjiu@ips.ac.cn
lab head
Shuangshuang Zhao
contact affiliation	Institut Pasteur of Shanghai
contact email	sszhao2@ips.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028785
     1. Label: PRIDE project
     2. Name: Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection