⮝ Full datasets listing

PXD028784

PXD028784 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePandanus amaryllifolius drought stress and recovery LC-MSMS TMT-Labelled
DescriptionThe aim of this project is to determine the protein changes of P. amaryllifolius in response to drought stress and during recovery. The protein changes between drought-stressed, well-watered, and recovered plants were evaluated using tandem mass tags (TMT)-based quantitative proteomics. The proteins were extracted from sample by using TCA/Acetone method and digested with trypsin. The digested proteins were then labelled with 10-plex TMT labelling prior fractionation with 12.5%, 17.5%, and 50% ACN with 0.1% triethylamine. The prepared proteins were then subjected to 1D data dependent acquisition (DDA) with nano LC column ionization source was set as positive ion mode, whereby 350-1850 m/z peptide precursors were scanned at 70,000 resolutions. The raw data generated was then searched against Viridiplantae protein sequences downloaded from UniProt. Of the 1,415 differentially abundant proteins, 74 were significantly altered. The majority of proteins differing between them were related to carbon metabolism, photosynthesis, stress response, and antioxidant activity. This is the first study that reports the protein changes in response to drought stress in Pandanus. The data generated provide an insight into the drought-responsive mechanisms in P. amaryllifolius.
HostingRepositoryPRIDE
AnnounceDate2022-02-16
AnnouncementXMLSubmission_2022-02-16_13:40:59.604.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBoon Chin Tan
SpeciesList scientific name: Pandanus amaryllifolius; NCBI TaxID: 260201;
ModificationListmethionine oxidation with neutral loss of 80 Da; TMT6plex-126 reporter+balance reagent acylated residue; carbamoylated residue; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-27 06:41:06ID requested
12022-02-16 13:41:00announced
Publication List
Amnan MAM, Aizat WM, Khaidizar FD, Tan BC, . Plants (Basel), 11(2):(2022) [pubmed]
Keyword List
submitter keyword: Pandanus amaryllifolius, abiotic stress, drought stress, recovery, TMT-labelled, LC-MSMS
Contact List
Boon Chin Tan
contact affiliationCentre for Research in Biotechnology for Agriculture(CEBAR),Universiti Malaya, Malaysia.
contact emailboonchin@um.edu.my
lab head
Boon Chin Tan
contact affiliationUniversiti Malaya (UM), CEBAR
contact emailboonchin@um.edu.my
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028784
PRIDE project URI
Repository Record List
[ + ]