PXD028784

PXD028784 is an original dataset announced via ProteomeXchange.

Title	Pandanus amaryllifolius drought stress and recovery LC-MSMS TMT-Labelled
Description	The aim of this project is to determine the protein changes of P. amaryllifolius in response to drought stress and during recovery. The protein changes between drought-stressed, well-watered, and recovered plants were evaluated using tandem mass tags (TMT)-based quantitative proteomics. The proteins were extracted from sample by using TCA/Acetone method and digested with trypsin. The digested proteins were then labelled with 10-plex TMT labelling prior fractionation with 12.5%, 17.5%, and 50% ACN with 0.1% triethylamine. The prepared proteins were then subjected to 1D data dependent acquisition (DDA) with nano LC column ionization source was set as positive ion mode, whereby 350-1850 m/z peptide precursors were scanned at 70,000 resolutions. The raw data generated was then searched against Viridiplantae protein sequences downloaded from UniProt. Of the 1,415 differentially abundant proteins, 74 were significantly altered. The majority of proteins differing between them were related to carbon metabolism, photosynthesis, stress response, and antioxidant activity. This is the first study that reports the protein changes in response to drought stress in Pandanus. The data generated provide an insight into the drought-responsive mechanisms in P. amaryllifolius.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_13:40:59.604.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Boon Chin Tan
SpeciesList	scientific name: Pandanus amaryllifolius; NCBI TaxID: 260201;
ModificationList	methionine oxidation with neutral loss of 80 Da; TMT6plex-126 reporter+balance reagent acylated residue; carbamoylated residue; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-27 06:41:06	ID requested
⏵ 1	2022-02-16 13:41:00	announced

Amnan MAM, Aizat WM, Khaidizar FD, Tan BC, . Plants (Basel), 11(2):(2022) [pubmed]

submitter keyword: Pandanus amaryllifolius, abiotic stress, drought stress, recovery, TMT-labelled, LC-MSMS

Boon Chin Tan
contact affiliation	Centre for Research in Biotechnology for Agriculture(CEBAR),Universiti Malaya, Malaysia.
contact email	boonchin@um.edu.my
lab head
Boon Chin Tan
contact affiliation	Universiti Malaya (UM), CEBAR
contact email	boonchin@um.edu.my
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028784
     1. Label: PRIDE project
     2. Name: Pandanus amaryllifolius drought stress and recovery LC-MSMS TMT-Labelled