PXD028781

PXD028781 is an original dataset announced via ProteomeXchange.

Title	Toxoplasma gondii MOB1 interactome
Description	MOB1 is a conserved protein that regulates cellular proliferation versus apoptosis, centrosome duplication and cellular differentiation in multicellular eukaryotes and also cytokinesis and division axis orientation in unicellular and multicellular eukaryotes. Toxoplasma gondii, an obligate intracellular parasite of veterinary and medical importance, presents one MOB1 protein. T. gondii interconverts between several cellular stages during its life cycle, namely between fast replicating tachyzoite and slow replicating bradyzoite stages during its asexual cycle, a key ability for its success as a parasite. Bradyzoites produce tissue cysts, establishing a chronic infection that enables recrudescence. Conversion is dependent on cell cycle regulation and involves cell differentiation and regulation of replication. This led us to select MOB1 as a strong candidate to be involved in the Toxoplasma replication process. To elucidate how MOB1 acts in T. gondii, we employed a proximity biotinylation method and identified the MOB1 interactome. Toxoplasma gondii RH tachyzoites were transfected with BirA containing plasmid vectors for random integration and two strains were isolated. The control strain expresses a FLAG-BirA recombinant protein while the test strain expresses a FLAG-BirA-MOB1 recombinant protein. Biotinylated proteins were purified using streptavidin-agarose beads. The purified proteins were trypsinized and analyzed by nanoLC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_13:40:02.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inês Delgado
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	biotinylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-27 03:37:40	ID requested
⏵ 1	2022-02-16 13:40:02	announced

Delgado ILS, Tavares A, Francisco S, Santos D, Coelho J, Basto AP, Z, ú, quete S, M, ü, ller J, Hemphill A, Meissner M, Soares H, Leit, ã, o A, Nolasco S, . Biology (Basel), 10(12):(2021) [pubmed]

submitter keyword: Toxoplasma gondii, MOB1, Hippo pathway, Mitotic exit network, BioID, proximity biotinylation, nanoLC-MS/MS

Alexandre Leitão
contact affiliation	CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisboa, Portugal
contact email	alexandre@fmv.ulisboa.pt
lab head
Inês Delgado
contact affiliation	FMV-UL, FMV-ULHT
contact email	ineslsdelgado@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028781
     1. Label: PRIDE project
     2. Name: Toxoplasma gondii MOB1 interactome