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PXD028769

PXD028769 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleN-terminome analyses of the intramembrane-cleaving protease SPPL3
DescriptionThe intramembrane protease SPPL3 can specifically cleave select Golgi enzymes, enabling their secretion and concomitantly altering global cellular glycosylation, yet the entire range of Golgi glycan-modifying enzymes cleaved by SPPL3 under physiological conditions remains to be defined. Here, we established isogenic SPPL3-deficient HEK293 and HeLa cell lines and applied N-terminomics to identify substrates cleaved by endogenous SPPL3 and released into conditioned cell culture supernatants.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:53:42.441.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Tholey
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-26 22:54:48ID requested
12022-03-17 06:39:57announced
22023-11-14 08:53:42announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: HEK293, Label-Free, HeLa, SPPL3, N-terminomics
Contact List
Andreas Tholey
contact affiliationSystematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Kiel University, 24105 Kiel, Germany.
contact emaila.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliationSystematic Proteome Research & Bioanalytics, University of Kiel
contact emaila.tholey@iem.uni-kiel.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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