PXD028769 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | N-terminome analyses of the intramembrane-cleaving protease SPPL3 |
Description | The intramembrane protease SPPL3 can specifically cleave select Golgi enzymes, enabling their secretion and concomitantly altering global cellular glycosylation, yet the entire range of Golgi glycan-modifying enzymes cleaved by SPPL3 under physiological conditions remains to be defined. Here, we established isogenic SPPL3-deficient HEK293 and HeLa cell lines and applied N-terminomics to identify substrates cleaved by endogenous SPPL3 and released into conditioned cell culture supernatants. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:53:42.441.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreas Tholey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion Lumos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-26 22:54:48 | ID requested | |
1 | 2022-03-17 06:39:57 | announced | |
⏵ 2 | 2023-11-14 08:53:42 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: HEK293, Label-Free, HeLa, SPPL3, N-terminomics |
Contact List
Andreas Tholey |
contact affiliation | Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Kiel University, 24105 Kiel, Germany. |
contact email | a.tholey@iem.uni-kiel.de |
lab head | |
Andreas Tholey |
contact affiliation | Systematic Proteome Research & Bioanalytics, University of Kiel |
contact email | a.tholey@iem.uni-kiel.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028769
- Label: PRIDE project
- Name: N-terminome analyses of the intramembrane-cleaving protease SPPL3