PXD028754

PXD028754 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparison of three enrichment methods of biotinylated peptides or proteins from AirID-CRBN-expressing MM1.S cells
Description	MM1.S cells stably expressing AGIA-AirID-CRBN were treated with DMSO or 10 µM pomalidomide in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. The cells were divided into three tubes and lysed. After tryptic digestion, biotinylated peptides were enriched using Tamavidin 2-REV magnetic beads or anti-biotin antibody beads. Biotinylated proteins were enriched using NanoLink streptavidin magnetic beads followed by digestion with Trypsin. The resultant peptides were analyzed by LC-MS/MS.
HostingRepository	jPOST
AnnounceDate	2021-11-06
AnnouncementXML	Submission_2022-09-18_02:50:05.037.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; N6-biotinyl-L-lysine; L-methionine sulfoxide
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-26 01:30:26	ID requested
1	2021-11-05 17:21:52	announced
2	2022-01-11 18:40:12	announced	2022-01-12: Updated PubMed.
⏵ 3	2022-09-18 02:50:05	announced	2022-09-18: Updated FTP location.

Keyword List

submitter keyword: biotinylation, Tamavidin 2-REV, anti-biotin antibody, streptavidin

submitter keyword: biotinylation, Tamavidin 2-REV, anti-biotin antibody, streptavidin

Contact List

Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001329/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001329/