PXD028733

PXD028733 is an original dataset announced via ProteomeXchange.

Title	A whole genome CRISPR screen identifies AHR loss as a mechanism of resistance to a PARP7 inhibitor
Description	Inhibitors directed towards PARP1 and PARP2 are approved agents for the treatment of BRCA-related cancers. Other members of the PARP family have also been implicated in cancer and are being assessed as therapeutic targets in cancer and other diseases. In fact, an inhibitor of PARP7 (RBN-2397) has now reached early-stage human clinical trials. Here, we performed a genome-wide CRISPR screen for genes that modify the response of cells to RBN-2397. We identify the polycyclic aromatic hydrocarbon receptor AHR and multiple components of the cohesin complex as determinants of resistance to this agent. Activators and inhibitors of AHR modulate the cellular response to PARP7 inhibition, suggesting potential combination therapy approaches.
HostingRepository	PRIDE
AnnounceDate	2022-04-06
AnnouncementXML	Submission_2022-05-06_07:03:40.542.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minkyu Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-23 22:55:35	ID requested
1	2022-04-06 09:12:27	announced
⏵ 2	2022-05-06 07:03:41	announced	2022-05-06: Updated publication reference for PubMed record(s): 35439318.

Chen H, Diolaiti ME, O'Leary PC, Rojc A, Krogan NJ, Kim M, Ashworth A, A Whole-Genome CRISPR Screen Identifies AHR Loss as a Mechanism of Resistance to a PARP7 Inhibitor. Mol Cancer Ther, 21(7):1076-1089(2022) [pubmed]

submitter keyword: Human, PARP7, TurboID

Nevan Krogan
contact affiliation	University of California San Francisco
contact email	minkyu.kim@ucsf.edu
lab head
Minkyu Kim
contact affiliation	University of California San Francisco
contact email	minkyu.kim@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD028733

PRIDE project URI

• PRIDE
  1. PXD028733
     1. Label: PRIDE project
     2. Name: A whole genome CRISPR screen identifies AHR loss as a mechanism of resistance to a PARP7 inhibitor