PXD028731

PXD028731 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A stress-reduced passaging technique improves the viability of human pluripotent cells
Description	Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications. Furthermore, the method did not alter the PSC characteristics after long-term culture and attenuated the growth advantage of abnormal subpopulations. This robust passaging method minimizes experimental error and improves the quality control of human PSC research and applications.
HostingRepository	jPOST
AnnounceDate	2022-01-23
AnnouncementXML	Submission_2022-11-20_17:58:30.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iwasaki Mio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-23 22:09:30	ID requested
1	2022-01-22 07:00:05	announced
2	2022-09-18 02:44:03	announced	2022-09-18: Updated FTP location.
⏵ 3	2022-11-20 17:58:31	announced	2022-11-21: Updated PubMed.

Publication List

Takahashi K, Okubo C, Nakamura M, Iwasaki M, Kawahara Y, Tabata T, Miyamoto Y, Woltjen K, Yamanaka S, A stress-reduced passaging technique improves the viability of human pluripotent cells. Cell Rep Methods, 2(2):100155(2022) [pubmed]

Takahashi K, Okubo C, Nakamura M, Iwasaki M, Kawahara Y, Tabata T, Miyamoto Y, Woltjen K, Yamanaka S, A stress-reduced passaging technique improves the viability of human pluripotent cells. Cell Rep Methods, 2(2):100155(2022) [pubmed]

Keyword List

submitter keyword: human pluripotent cells、DIA

submitter keyword: human pluripotent cells、DIA

Contact List

Mio Iwasaki
lab head
Iwasaki Mio
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001327/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001327/