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PXD028704-1

PXD028704 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMaster mitotic kinases regulate viral genome delivery during papillomavirus cell entry
DescriptionCell cycle progression into mitosis induce cellular rearrangements such as mitotic spindle formation, Golgi fragmentation, and nuclear envelope breakdown. Like certain retroviruses, nuclear delivery of HPV16 genomes is facilitated by these processes during entry into host cells by tethering of the viral DNA to mitotic chromosomes through the minor capsid protein L2. However, the mechanism of delivery onto and tethering to the condensed chromosomes is barely understood on a mechanistic level. To date it is unclear, which cellular proteins facilitate this process in interaction with L2 or how this process is regulated. Here, we discovered that HPV16 minor capsid protein L2 is phosphorylated during entry upon mitosis onset on conserved residues within the chromosome-binding region (CBR) that is responsible for nuclear import. The crucial L2 phosphorylations occurred sequentially by the master mitotic kinases CDK1 and PLK1. L2 phosphorylation, thus, not only regulated timely delivery of HPV16 vDNA to mitotic chromatin at mitosis onset, but also likely resulted in a conformational switch in L2 that allowed engagement of cellular proteins for this purpose. In summary, our work demonstrates for the first time a crucial role of mitotic kinases for nuclear entry of a DNA virus and provides important insights into the molecular mechanism of pathogen import into the nucleus during mitosis.
HostingRepositoryPRIDE
AnnounceDate2022-12-22
AnnouncementXMLSubmission_2022-12-22_03:19:01.084.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHannesDrexler
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human papillomavirus type 16; NCBI TaxID: 333760;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-22 12:10:29ID requested
12022-12-22 03:19:01announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MSMS, HPV, proteomics, CDK1, mitosis, human papillomavirus, PLK1,Virus entry, nuclear import
Contact List
Hannes C. A.Drexler
contact affiliationMax Planck Institute for Moleclar Biomedicine Bioanalyticyl Mass Spectrometry Unit Röntgenstr. 20 48149 Münster Germany
contact emailhannes.drexler@mpi-muenster.mpg.de
lab head
HannesDrexler
contact affiliationBioanalytical Mass Spectrometry
contact emailhannes.drexler@mpi-muenster.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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