PXD028687

PXD028687 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A conserved nucleotide-dependent metal chaperone for managing the cellular zinc economy during zinc limitation
Description	How cells safeguard essential zinc-dependent functions during zinc deficiency is poorly understood. A long-debated strategy is whether soluble metal-trafficking chaperones exist to prioritize specific zinc-dependent proteins. We identified a eukaryotic family of metallochaperones that physically interacts with zinc-dependent methionine aminopeptidase type I (MAP1) in human and yeast. Deletion of the yeast metallochaperone-encoding gene NMC1 (formerly YNR029c) leads to a zinc-deficiency growth defect and defective initiator methionine cleavage caused by loss of Map1p activity. To better understand the observed fitness defects due to the lack of NMC1 under zinc deficiency, we used proteomics with Tandem Mass Tag (TMT) quantitation derived from WT, nmc1Delta, and map2Delta nmc1Delta strains grown in zinc-limited (1 uM) or zinc-replete (100 uM) conditions. Proteomics reveal global impacts due to the loss of NMC1 and Map1p function, including mis-regulation of the Zap1p regulon, and suggests that Nmc1p is required to avoid a compounding effect of Map1p dysfunction on cell survival during zinc deficiency.
HostingRepository	MassIVE
AnnounceDate	2022-08-16
AnnouncementXML	Submission_2022-08-16_09:30:57.707.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationList	TMTpro; Carbamidomethyl; Oxidation
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-21 13:54:10	ID requested
⏵ 1	2022-08-16 09:30:58	announced

Publication List

Pasquini M, Grosjean N, Hixson KK, Nicora CD, Yee EF, Lipton M, Blaby IK, Haley JD, Blaby-Haas CE, Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase. Cell Rep, 39(7):110834(2022) [pubmed]

Pasquini M, Grosjean N, Hixson KK, Nicora CD, Yee EF, Lipton M, Blaby IK, Haley JD, Blaby-Haas CE, Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase. Cell Rep, 39(7):110834(2022) [pubmed]

Keyword List

submitter keyword: MetAP, GTPase, zinc starvation, nutrient limitation

submitter keyword: MetAP, GTPase, zinc starvation, nutrient limitation

Contact List

Crysten Blaby-Haas
contact affiliation	Brookhaven National Laboratory
contact email	cblaby@bnl.gov
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088135/